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キーワード:
HCO−3; Stimulated ATPase; Rabbit Submandibular Gland
要旨:
An ATPase stimulated by HCO−3 -ions and inhibited by SCN-ions could be found in glandula submandibularis of rabbit with following properties: 1. It is stimulated by a variety of oxybases according to a general base catalytic mechanism. 2. It is inhibited by both SCN−-and OCN−-ions. 3. It can be solubilized by Triton X 100 hereby yielding a marked increase in the activity response to HCO−3 -and SCN−-ions. 4. It can be distinguished from mitochondrial ATPase because of the different behaviour of this enzyme and mitochondrial marker such as succinic dehydrogenase using sucrose gradient centrifugations.
This enzyme resembles closely an enzyme preparation which had been found in plasma membranes of gastric mucosa and pancreatic tissue and which is assumed to be involved in H+/HCO−3 transport system of these organs. The presence of HCO−3 ATPase in glandula submandibularis indicates that a similar H+/HCO−3 transport-mechanism may be present in this organ like in gastric mucosa and pancreatic tissue.
