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  Cooperativity of membrane-protein and protein–protein interactions control membrane remodeling by epsin 1 and affects clathrin-mediated endocytosis

Kroppen, B., Teske, N., Yambire, K. F., Denkert, N., Mukherjee, I., Tarasenko, D., et al. (2021). Cooperativity of membrane-protein and protein–protein interactions control membrane remodeling by epsin 1 and affects clathrin-mediated endocytosis. Cellular and Molecular Life Sciences, 78(5), 2355-2370. doi:10.1007/s00018-020-03647-z.

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 Creators:
Kroppen, B., Author
Teske, N., Author
Yambire, K. F., Author
Denkert, N., Author
Mukherjee, I., Author
Tarasenko, D., Author
Jaipuria, G., Author
Zweckstetter, M.1, Author              
Milosevic, I., Author
Steinem, C., Author
Meinecke, M., Author
Affiliations:
1Research Group of Protein Structure Determination using NMR, MPI for biophysical chemistry, Max Planck Society, ou_578571              

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Free keywords: Membrane curvature; Membrane dynamics; ENTH domain; Clathrin-mediated endocytosis; Reconstitution of membrane dynamics
 Abstract: Membrane remodeling is a critical process for many membrane trafficking events, including clathrin-mediated endocytosis. Several molecular mechanisms for protein-induced membrane curvature have been described in some detail. Contrary, the effect that the physico-chemical properties of the membrane have on these processes is far less well understood. Here, we show that the membrane binding and curvature-inducing ENTH domain of epsin1 is regulated by phosphatidylserine (PS). ENTH binds to membranes in a PI(4,5)P2-dependent manner but only induces curvature in the presence of PS. On PS-containing membranes, the ENTH domain forms rigid homo-oligomers and assembles into clusters. Membrane binding and membrane remodeling can be separated by structure-to-function mutants. Such oligomerization mutants bind to membranes but do not show membrane remodeling activity. In vivo, they are not able to rescue defects in epidermal growth factor receptor (EGFR) endocytosis in epsin knock-down cells. Together, these data show that the membrane lipid composition is important for the regulation of protein-dependent membrane deformation during clathrin-mediated endocytosis.

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Language(s): eng - English
 Dates: 2020-09-302021-03
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/s00018-020-03647-z
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Project name : SFB 803
Grant ID : -
Funding program : Project A11
Funding organization : Deutsche Forschungsgemeinschaft

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Title: Cellular and Molecular Life Sciences
Source Genre: Journal
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Pages: - Volume / Issue: 78 (5) Sequence Number: - Start / End Page: 2355 - 2370 Identifier: -