English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Chondroitin Sulfate Disaccharides in the Gas Phase: Differentiation and Conformational Constraints

Lettow, M., Greis, K., Grabarics, M., Horlebein, J., Miller, R. L., Meijer, G., et al. (2021). Chondroitin Sulfate Disaccharides in the Gas Phase: Differentiation and Conformational Constraints. The Journal of Physical Chemistry A, 125(20), 4373-4379. doi:10.1021/acs.jpca.1c02463.

Item is

Files

show Files
hide Files
:
acs.jpca.1c02463.pdf (Publisher version), 905KB
Name:
acs.jpca.1c02463.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
2021
Copyright Info:
The Author(s)

Locators

show

Creators

show
hide
 Creators:
Lettow, Maike1, 2, Author           
Greis, Kim1, 2, Author           
Grabarics, Márkó1, 2, Author           
Horlebein, Jan1, 2, Author           
Miller, Rebecca L.3, Author
Meijer, Gerard1, Author           
Helden, Gert von1, Author           
Pagel, Kevin1, 2, Author           
Affiliations:
1Molecular Physics, Fritz Haber Institute, Max Planck Society, ou_634545              
2Institut für Chemie und Biochemie, Freie Universität Berlin, 14195 Berlin, Germany, ou_persistent22              
3Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, DK-2200 Copenhagen N, Denmark, ou_persistent22              

Content

show
hide
Free keywords: Anions,Carbohydrates,Ions,Energy,Molecular structure
 Abstract: Glycosaminoglycans (GAGs) are a family of complex carbohydrates vital to all mammalian organisms and involved in numerous biological processes. Chondroitin and dermatan sulfate, an important class of GAGs, are linear macromolecules consisting of disaccharide building blocks of N-acetylgalactosamine and two different uronic acids. The varying degree and the site of sulfation render their characterization challenging. Here, we combine mass spectrometry with cryogenic infrared spectroscopy in the wavenumber range from 1000 to 1800 cm-1. Fingerprint spectra were recorded for a comprehensive set of disaccharides bearing all known motifs of sulfation. In addition, state-of-the-art quantum chemical calculations were performed to aid the understanding of the differences in the experimental fingerprint spectra. The results show that the degree and position of charged sulfate groups define the size of the conformational landscape in the gas phase. The detailed understanding of cryogenic infrared spectroscopy for acidic and often highly sulfated glycans may pave the way to utilize the technique in fragment-based sequencing approaches.

Details

show
hide
Language(s): eng - English
 Dates: 2021-03-182021-05-122021-05-27
 Publication Status: Issued
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acs.jpca.1c02463
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Journal of Physical Chemistry A
  Other : J. Phys. Chem. A
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Columbus, OH : American Chemical Society
Pages: 7 Volume / Issue: 125 (20) Sequence Number: - Start / End Page: 4373 - 4379 Identifier: ISSN: 1089-5639
CoNE: https://pure.mpg.de/cone/journals/resource/954926947766_4