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  Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization

Nikolay, R., Hilal, T., Schmidt, S., Qin, B., Schwefel, D., Vieira-Vieira, C. H., et al. (2021). Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization. Molecular Cell, 81(6), 1200-1215. doi:10.1016/j.molcel.2021.02.006.

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Nikolay, Rainer , Author
Hilal, Tarek , Author
Schmidt, Sabine , Author
Qin, Bo, Author
Schwefel, David, Author
Vieira-Vieira, Carlos H. , Author
Mielke, Thorsten1, Author              
Bürger, Jörg1, 2, Author              
Affiliations:
1Microscopy and Cryo-Electron Microscopy (Head: Thorsten Mielke), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              
2Institut für Medizinische Physik und Biophysik, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany, ou_persistent22              

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Free keywords: 50S subunit; ObgE; RluD; RsfS; YjgA; cryo-electron microscopy; ribosome; ribosome assembly; ribosome biogenesis; structure
 Abstract: Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. In this study, we analyze physiologic prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits from an Escherichia coli strain with the biogenesis factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS, and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.

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Language(s): eng - English
 Dates: 2021-02-262021-03-18
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.molcel.2021.02.006
 Degree: -

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Title: Molecular Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: 16 Volume / Issue: 81 (6) Sequence Number: - Start / End Page: 1200 - 1215 Identifier: ISSN: 1097-2765
CoNE: https://pure.mpg.de/cone/journals/resource/954925610929