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  Quantitative Assessment of Labeling Probes for Super-Resolution Microscopy Using Designer DNA Nanostructures

Ganji, M., Schlichthaerle, T., Eklund, A. S., Strauss, S., & Jungmann, R. (2021). Quantitative Assessment of Labeling Probes for Super-Resolution Microscopy Using Designer DNA Nanostructures. ChemPhysChem, 22, 911-914. doi:10.1002/cphc.202100185.

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Genre: Journal Article

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 Creators:
Ganji, Mahipal1, Author              
Schlichthaerle, Thomas1, Author              
Eklund, Alexandra S.1, Author              
Strauss, Sebastian1, Author
Jungmann, Ralf1, Author              
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              

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Free keywords: Chemistry; Physics; DNA origami; DNA-PAINT; labeling probes; single-molecule imaging; super-resolution microscopy;
 Abstract: Improving labeling probes for state-of-the-art super-resolution microscopy is becoming of major importance. However, there is currently a lack of tools to quantitatively evaluate probe performance regarding efficiency, precision, and achievable resolution in an unbiased yet modular fashion. Herein, we introduce designer DNA origami structures combined with DNA-PAINT to overcome this issue and evaluate labeling efficiency, precision, and quantification using antibodies and nanobodies as exemplary labeling probes. Whereas current assessment of binders is mostly qualitative, e. g. based on an expected staining pattern, we herein present a quantitative analysis platform of the antigen labeling efficiency and achievable resolution, allowing researchers to choose the best performing binder. The platform can furthermore be readily adapted for discovery and precise quantification of a large variety of additional labeling probes.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000646710200001
DOI: 10.1002/cphc.202100185
 Degree: -

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Title: ChemPhysChem
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 22 Sequence Number: - Start / End Page: 911 - 914 Identifier: ISSN: 1439-4235
CoNE: https://pure.mpg.de/cone/journals/resource/954925409790