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  Mechanisms involved in small intestinal transport of calcium and phosphate ions: studies with isolated plasma membrane vesicles

Hildmann, B., Storelli, C., Schmidt, A., & Murer, H. (1981). Mechanisms involved in small intestinal transport of calcium and phosphate ions: studies with isolated plasma membrane vesicles. Oxford, New York: Pergamon Press.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-9253-5 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-9254-4
Genre: Conference Paper

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 Creators:
Hildmann, Bruno1, Author           
Storelli, Carlo1, Author           
Schmidt, A.1, Author           
Murer, Heini2, Author
Affiliations:
1Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              
2Institut für physiologische Chemie, Universität Freiburg, CH-1700 Freiburg, Switzerland, ou_persistent22              

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 Abstract: Publisher Summary: This chapter examines mechanisms involved in small intestinal transport of calcium and phosphate ions. In an experiment described in the chapter, brush border membranes (BBM) were isolated by an Mg++-precipitation method from rabbit duodenum. Basal–lateral plasma membranes (BLM) were isolated by a Percoll centrifugation method from rat upper small intestine. Transport studies were carried out by a rapid filtration technique. When Ca-transport was studied, membrane samples were washed in 1 ml of ice-cold buffer solution containing 1 mmol/l EGTA prior to filtration to remove Ca bound to the outside of membrane vesicles. All incubations were carried out at 25°C and pH 7.4. It was concluded that for Pi, the Na–Pi-cotransport system in the brush border membrane is a site of coupling of Pi-transport to the energy of the Na-gradient and a site of regulation of Pi-transport by 1,25-D3. Mechanisms for active transport of Ca are localized in the BLM: (1) an ATP-driven system and (2) a Na/Ca exchange system. Regulation of Ca permeability by 1,25-D3 can be demonstrated at the BBM.

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Language(s): eng - English
 Dates: 1981
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/B978-0-08-026825-5.50070-X
 Degree: -

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Title: Proceedings of the 28th International Congress of Physiological Sciences
Place of Event: Budapest, Hungary
Start-/End Date: 1980 - 1980
Invited: Yes

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Title: Advances in Physiological Sciences
  Subtitle : Nutrition, Digestion, Metabolism
Source Genre: Series
 Creator(s):
Gáti, T.1, Editor
Szollár, L.G1, Editor
Ungváry, Gy.1, Editor
Affiliations:
1 Budapest, Hungary, ou_persistent22            
Publ. Info: Oxford, New York : Pergamon Press
Pages: 553 Volume / Issue: 12 Sequence Number: - Start / End Page: 459 - 463 Identifier: DOI: 10.1016/C2013-0-05999-3
ISBN: 978-0-08-026825-5