English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Further Studies on the Potential Role of an Anion-Stimulated Mg-ATPase in Rat Proximal Tubule Proton Transport

Kinne-Saffran, E., & Kinne, R. (1980). Further Studies on the Potential Role of an Anion-Stimulated Mg-ATPase in Rat Proximal Tubule Proton Transport. In F. Bronner, & A. Kleinzeller (Eds.), Current Topics in Membranes and Transport (pp. 337-341). Academic Press Inc. doi:10.1016/S0070-2161(08)60292-0.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Kinne-Saffran, Evamaria1, Author           
Kinne, Rolf1, Author           
Affiliations:
1Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              

Content

show
hide
Free keywords: -
 Abstract: Publisher Summary: This chapter discusses the problem of plasma membrane-bound Mg-adenosine triphosphatase (ATPase) in rat kidney and experimentally proves the existence of an anion-sensitive Mg2+- ATPase in brush border membranes from rat kidney cortex. It is possible to differentiate between this enzyme and a mitochondrial Mg-ATPase from the same tissue using substances such as atractyloside and filipin that interact exclusively with mitochondria or brush border membranes. Parallel studies on Mg-ATPase activity of mitochondrial and brush border fractions reveal that the enzyme in both fractions is stimulated by oxyanions—such as sulfite and bicarbonate—and is inhibited by aurovertin and oligomycin. The mitochondrial ATPase activity shows a higher sensitivity to the stimulators and inhibitors with a difference in the response of Mg-ATPase activity of the two fractions that has been obtained using atractyloside—an inhibitor of the mitochondrial adenine nucleotide translocator—and filipin—an antibiotic interacting with cholesterol in the brush border membrane. The use of filipin might provide a tool to further investigate the relationship between brush border Mg-ATPase and protein translocation in vitro and in vivo.

Details

show
hide
Language(s): eng - English
 Dates: 2008-05-301980-12
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/S0070-2161(08)60292-0
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Current Topics in Membranes and Transport
  Subtitle : Cellular Mechanisms of Renal Tubular Ion Transport
Source Genre: Series
 Creator(s):
Bronner, Felix, Editor
Kleinzeller, Arnost, Editor
Affiliations:
-
Publ. Info: Academic Press Inc.
Pages: 433 Volume / Issue: 13 Sequence Number: - Start / End Page: 337 - 341 Identifier: ISSN: 0070-2161
ISBN: 978-0-12-153313-7