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  C4-dicarboxylates and l-aspartate utilization by Escherichia coli K-12 in the mouse intestine: l-aspartate as a major substrate for fumarate respiration and as a nitrogen source

Schubert, C., Winter, M., Ebert-Jung, A., Kierszniowska, S., Nagel-Wolfrum, K., Schramm, T., et al. (2021). C4-dicarboxylates and l-aspartate utilization by Escherichia coli K-12 in the mouse intestine: l-aspartate as a major substrate for fumarate respiration and as a nitrogen source. Environmental Microbiology, 23(5), 2564-2577. doi:10.1111/1462-2920.15478.

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https://doi.org/10.1111/1462-2920.15478 (Verlagsversion)
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 Urheber:
Schubert, Christopher, Autor
Winter, Maria, Autor
Ebert-Jung, Andrea, Autor
Kierszniowska, Sylwia, Autor
Nagel-Wolfrum, Kerstin, Autor
Schramm, Thorben1, 2, Autor           
Link, Hannes1, 2, Autor           
Winter, Sebastian, Autor
Unden, Gottfried, Autor
Affiliations:
1Emmy Noether Research Group Dynamic Control of Metabolic Networks, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266292              
2Max Planck Institute for Terrestrial Microbiology, Max Planck Society, Karl-von-Frisch-Strasse 10, D-35043 Marburg, DE, ou_3135468              

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 Zusammenfassung: C4-dicarboxylates, such as fumarate, l-malate and l-aspartate represent
substrates for anaerobic growth of Escherichia coli by fumarate
respiration. Here, we determined whether C4-dicarboxylate metabolism, as
well as fumarate respiration, contribute to colonization of the
mammalian intestinal tract. Metabolite profiling revealed that the
murine small intestine contained high and low levels of l-aspartate and
l-malate respectively, whereas fumarate was nearly absent. Under
laboratory conditions, addition of C4-dicarboxylate at concentrations
corresponding to the levels of the C4-dicarboxylates in the small
intestine (2.6 mmol kg(-1) dry weight) induced the dcuBp-lacZ reporter
gene (67% of maximal) in a DcuS-DcuR-dependent manner. In addition to
its role as a precursor for fumarate respiration, l-aspartate was able
to supply all the nitrogen required for anaerobically growing E. coli.
DcuS-DcuR-dependent genes were transcribed in the murine intestine, and
mutants with defective anaerobic C4-dicarboxylate metabolism (dcuSR,
frdA, dcuB, dcuA and aspA genes) were impaired for colonizing the murine
gut. We conclude that l-aspartate plays an important role in providing
fumarate for fumarate respiration and supplying nitrogen for E. coli in
the mouse intestine.

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Sprache(n): eng - English
 Datum: 2021-05
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000646705600001
DOI: 10.1111/1462-2920.15478
 Art des Abschluß: -

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Titel: Environmental Microbiology
  Andere : Environmental Microbiology and Environmental Microbiology Reports
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Oxford, England : Blackwell Science
Seiten: - Band / Heft: 23 (5) Artikelnummer: - Start- / Endseite: 2564 - 2577 Identifikator: ISSN: 1462-2912
CoNE: https://pure.mpg.de/cone/journals/resource/959328105031