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  High cell densities favor lysogeny: induction of an H20 prophage is repressed by quorum sensing and enhances biofilm formation in Vibrio anguillarum

Tan, D., Hansen, M. F., de Carvalho, L. N., Roder, H. L., Burmolle, M., Middelboe, M., & Svenningsen, S. L. (2020). High cell densities favor lysogeny: induction of an H20 prophage is repressed by quorum sensing and enhances biofilm formation in Vibrio anguillarum. ISME JOURNAL, 14(7), 1731-1742. doi:10.1038/s41396-020-0641-3.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-0008-BE92-D 版のパーマリンク: https://hdl.handle.net/21.11116/0000-0008-F236-A
資料種別: 学術論文

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 作成者:
Tan, Demeng1, 著者
Hansen, Mads Frederik2, 著者
de Carvalho, Luis Nunes1, 著者
Roder, Henriette Lyng1, 著者
Burmolle, Mette1, 著者
Middelboe, Mathias1, 著者
Svenningsen, Sine Lo1, 著者
所属:
1external, ou_persistent22              
2Max Planck Research Group Bacterial Biofilms, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, Karl-von-Frisch-Strasse 10, D-35043 Marburg, DE, ou_3266298              

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 要旨: Temperate phi H20-like phages are repeatedly identified at
geographically distinct areas as free phage particles or as prophages of
the fish pathogen Vibrio anguillarum. We studied mutants of a lysogenic
isolate of V. anguillarum locked in the quorum-sensing regulatory modes
of low (Delta vanT) and high (Delta vanO) cell densities by in-frame
deletion of key regulators of the quorum-sensing pathway. Remarkably, we
find that induction of the H20-like prophage is controlled by the
quorum-sensing state of the host, with an eightfold increase in phage
particles per cell in high-cell-density cultures of the
quorum-sensing-deficient Delta vanT mutant. Comparative studies with
prophage-free strains show that biofilm formation is promoted at low
cell density and that the H20-like prophage stimulates this behavior. In
contrast, the high-cell-density state is associated with reduced
prophage induction, increased proteolytic activity, and repression of
biofilm. The proteolytic activity may dually function to disperse the
biofilm and as a quorum-sensing-mediated antiphage strategy. We
demonstrate an intertwined regulation of phage-host interactions and
biofilm formation, which is orchestrated by host quorum-sensing
signaling, suggesting that increased lysogeny at high cell density is
not solely a strategy for phages to piggy-back the successful bacterial
hosts but is also a host strategy evolved to take control of the
lysis-lysogeny switch to promote host fitness.

資料詳細

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 日付: 2020-07
 出版の状態: 出版
 ページ: -
 出版情報: -
 目次: -
 査読: -
 識別子（DOI, ISBNなど）: ISI: 000524892900002
DOI: 10.1038/s41396-020-0641-3
 学位: -

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出版物 1

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出版物名: ISME JOURNAL
種別: 学術雑誌
 著者・編者:
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出版社, 出版地: -
ページ: - 巻号: 14 (7) 通巻号: - 開始・終了ページ: 1731 - 1742 識別子（ISBN, ISSN, DOIなど）: ISSN: 1751-7362