ausblenden:
Schlagwörter:
-
Zusammenfassung:
Due to their biotechnological relevance as well as their importance as
disease agents, filamentous fungi and oomycetes have been prime
candidates for genetic selection and in vitro manipulation for decades.
With the advent of new genome editing technologies such manipulations
have reached a new level of speed and sophistication. The CRISPR-Cas9
genome editing technology in particular has revolutionized the ways how
desired mutations can be introduced. To date, the CRISPR-Cas9 genome
editing system has been established in more than 40 different species of
filamentous fungi and oomycetes. In this review we describe the various
approaches taken to assure expression of the components necessary for
editing and describe the varying strategies used to achieve gene
disruptions, gene replacements and precise editing. We discuss potential
problems faced when establishing the system, propose ways to circumvent
them and suggest future approaches not yet realized in filamentous fungi
or oomycetes.
