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  Targeted Metatranscriptomics of Soil Microbial Communities with Stable Isotope Probing

Hu, A., Lu, Y., Garcia, M. H., & Dumont, M. G. (2019). Targeted Metatranscriptomics of Soil Microbial Communities with Stable Isotope Probing. In STABLE ISOTOPE PROBING: METHODS AND PROTOCOLS (pp. 163-174).

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-BF5C-B Version Permalink: https://hdl.handle.net/21.11116/0000-0008-BF5D-A
Genre: Conference Paper

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 Creators:
Hu, Ang1, Author
Lu, Yahai2, Author           
Garcia, Marcela Hernandez2, Author
Dumont, Marc G.2, Author           
Affiliations:
1external, ou_persistent22              
2Department of Biogeochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266312              

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 Abstract: Metatranscriptomics is a powerful tool for capturing gene expression
patterns in microbial communities and investigating their responses to
environmental conditions. Stable isotope probing (SIP) is a method to
target specific functional groups of microorganisms in environmental
samples. The combination of RNA-SIP with metatranscriptomic analysis
enhances the detection and identification of mRNA from target
microorganisms. In this chapter we provide a protocol for RNA-SIP, mRNA
enrichment, and mRNA preparation for high-throughput sequencing using an
example of targeting methanotrophs in rice field soil.

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 Dates: 2019
 Publication Status: Issued
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 Rev. Type: -
 Identifiers: ISI: 000517291000014
DOI: 10.1007/978-1-4939-9721-3_13
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Title: STABLE ISOTOPE PROBING: METHODS AND PROTOCOLS
Source Genre: Proceedings
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Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 163 - 174 Identifier: ISSN: 1064-3745
ISBN: 978-1-4939-9721-3; 978-1-4939-9720-6

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Title: Methods in Molecular Biology
Source Genre: Series
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Pages: - Volume / Issue: 2046 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745