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  Loss of inner-envelope K+/H+ exchangers impairs plastid rRNA maturation and gene expression

Ann DeTar, R., Barahimipour, R., Manavski, N., Schwenkert, S., Höhner, R., Bölter, B., et al. (2021). Loss of inner-envelope K+/H+ exchangers impairs plastid rRNA maturation and gene expression. The Plant Cell, 33(7), 2479-2505. doi:10.1093/plcell/koab123.

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Ann DeTar, Rachael1, Author
Barahimipour, R.2, Author              
Manavski, Nikolay1, Author
Schwenkert, Serena1, Author
Höhner, Ricarda1, Author
Bölter, Bettina1, Author
Inaba, Takehito1, Author
Meurer, Jörg1, Author
Zoschke, R.2, Author              
Kunz, Hans-Henning1, Author
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1External Organizations, ou_persistent22              
2Translational Regulation in Plants, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2324691              

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 Abstract: The inner-envelope K+ EFFLUX ANTIPORTERS (KEA) 1 and 2 are critical for chloroplast development, ion homeostasis, and photosynthesis. However, the mechanisms by which changes in ion flux across the envelope affect organelle biogenesis remained elusive. Chloroplast development requires intricate coordination between the nuclear genome and the plastome. Many mutants compromised in plastid gene expression (PGE) display a virescent phenotype, i.e. delayed greening. The phenotypic appearance of Arabidopsis thaliana kea1 kea2 double mutants fulfills this criterion, yet a link to PGE has not been explored. Here, we show that a simultaneous loss of KEA1 and KEA2 results in maturation defects of the plastid ribosomal RNAs. This may be caused by secondary structure changes of rRNA transcripts and concomitant reduced binding of RNA-processing proteins, which we documented in the presence of skewed ion homeostasis in kea1 kea2. Consequently, protein synthesis and steady-state levels of plastome-encoded proteins remain low in mutants. Disturbance in PGE and other signs of plastid malfunction activate GUN1-dependent retrograde signaling in kea1 kea2, resulting in a dramatic downregulation of GOLDEN2-LIKE transcription factors to halt expression of photosynthesis-associated nuclear-encoded genes (PhANGs). PhANG suppression delays the development of fully photosynthesizing kea1 kea2 chloroplasts, probably to avoid progressing photo‐oxidative damage. Overall, our results reveal that KEA1/KEA2 function impacts plastid development via effects on RNA-metabolism and PGE.

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Language(s): eng - English
 Dates: 2021-06
 Publication Status: Published in print
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 Identifiers: DOI: 10.1093/plcell/koab123
BibTex Citekey: 10.1093/plcell/koab123
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Title: The Plant Cell
  Abbreviation : Plant C
Source Genre: Journal
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Publ. Info: Rockville : American Society of Plant Physiologists
Pages: - Volume / Issue: 33 (7) Sequence Number: - Start / End Page: 2479 - 2505 Identifier: ISSN: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X