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  Hepatitis C virus E2 envelope glycoprotein produced in Nicotiana benthamiana triggers humoral response with virus-neutralizing activity in vaccinated mice

Dobrica, M.-O., van Eerde, A., Tucureanu, C., Onu, A., Paruch, L., Caras, I., et al. (2021). Hepatitis C virus E2 envelope glycoprotein produced in Nicotiana benthamiana triggers humoral response with virus-neutralizing activity in vaccinated mice. Plant Biotechnology Journal. doi:10.1111/pbi.13631.

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Genre: Journal Article
Alternative Title : Plant Biotechnology Journal

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 Creators:
Dobrica, Mihaela-Olivia1, Author
van Eerde, André1, Author
Tucureanu, Catalin1, Author
Onu, Adrian1, Author
Paruch, Lisa1, Author
Caras, Iuliana1, Author
Vlase, Ene1, Author
Steen, Hege1, Author
Haugslien, Sissel1, Author
Alonzi, Dominic1, Author
Zitzmann, Nicole1, Author
Bock, R.2, Author              
Dubuisson, Jean1, Author
Popescu, Costin-Ioan1, Author
Stavaru, Crina1, Author
Liu Clarke, Jihong1, Author
Branza-Nichita, Norica1, Author
Affiliations:
1external, ou_persistent22              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Free keywords: plant biopharmaceuticals, HCV vaccine, E2 glycoprotein, N-glycosylation, HCV neutralizing antibodies
 Abstract: Summary Chronic infection with hepatitis C virus (HCV) remains a leading cause of liver-related pathologies and a global health problem, currently affecting more than 71 million people worldwide. The development of a prophylactic vaccine is much needed to complement the effective antiviral treatment available and achieve HCV eradication. Current strategies focus on increasing the immunogenicity of the HCV envelope glycoprotein E2, the major target of virus neutralizing antibodies, by testing various expression systems or manipulating the protein conformation and the N-glycosylation pattern. Here we report the first evidence of successful production of the full-length HCV E2 glycoprotein in Nicotiana benthamiana, by using the Agrobacterium-mediated transient expression technology. Molecular and functional analysis showed that the viral protein was correctly processed in plant cells and achieved the native folding required for binding to CD81, one of the HCV receptors. N-glycan analysis of HCV-E2 produced in N. benthamiana and mammalian cells indicated host-specific trimming of mannose residues and possibly, protein trafficking. Notably, the plant-derived viral antigen triggered a significant immune response in vaccinated mice, characterized by the presence of antibodies with HCV neutralizing activity. Together, our study demonstrates that N. benthamiana is a viable alternative to costly mammalian cell cultures for the expression of complex viral antigens and supports the use of plants as cost-effective production platforms for development of HCV vaccines.

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Language(s): eng - English
 Dates: 2021-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1111/pbi.13631
 Degree: -

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Title: Plant Biotechnology Journal
  Other : Plant Biotechnol. J.
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Pub.
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1467-7644
CoNE: https://pure.mpg.de/cone/journals/resource/110978984569611