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  Hepatitis C virus E2 envelope glycoprotein produced in Nicotiana benthamiana triggers humoral response with virus-neutralizing activity in vaccinated mice

Dobrica, M.-O., van Eerde, A., Tucureanu, C., Onu, A., Paruch, L., Caras, I., Vlase, E., Steen, H., Haugslien, S., Alonzi, D., Zitzmann, N., Bock, R., Dubuisson, J., Popescu, C.-I., Stavaru, C., Liu Clarke, J., & Branza-Nichita, N. (2021). Hepatitis C virus E2 envelope glycoprotein produced in Nicotiana benthamiana triggers humoral response with virus-neutralizing activity in vaccinated mice. Plant Biotechnology Journal, 19(10), 2027-2039. doi:10.1111/pbi.13631.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-0008-9CAA-9 版のパーマリンク: https://hdl.handle.net/21.11116/0000-000D-EECB-3
資料種別: 学術論文
その他のタイトル : Plant Biotechnology Journal

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 作成者:
Dobrica, Mihaela-Olivia1, 著者
van Eerde, André1, 著者
Tucureanu, Catalin1, 著者
Onu, Adrian1, 著者
Paruch, Lisa1, 著者
Caras, Iuliana1, 著者
Vlase, Ene1, 著者
Steen, Hege1, 著者
Haugslien, Sissel1, 著者
Alonzi, Dominic1, 著者
Zitzmann, Nicole1, 著者
Bock, R.2, 著者           
Dubuisson, Jean1, 著者
Popescu, Costin-Ioan1, 著者
Stavaru, Crina1, 著者
Liu Clarke, Jihong1, 著者
Branza-Nichita, Norica1, 著者
所属:
1external, ou_persistent22              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

内容説明

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キーワード: plant biopharmaceuticals, HCV vaccine, E2 glycoprotein, N-glycosylation, HCV neutralizing antibodies
 要旨: Summary Chronic infection with hepatitis C virus (HCV) remains a leading cause of liver-related pathologies and a global health problem, currently affecting more than 71 million people worldwide. The development of a prophylactic vaccine is much needed to complement the effective antiviral treatment available and achieve HCV eradication. Current strategies focus on increasing the immunogenicity of the HCV envelope glycoprotein E2, the major target of virus neutralizing antibodies, by testing various expression systems or manipulating the protein conformation and the N-glycosylation pattern. Here we report the first evidence of successful production of the full-length HCV E2 glycoprotein in Nicotiana benthamiana, by using the Agrobacterium-mediated transient expression technology. Molecular and functional analysis showed that the viral protein was correctly processed in plant cells and achieved the native folding required for binding to CD81, one of the HCV receptors. N-glycan analysis of HCV-E2 produced in N. benthamiana and mammalian cells indicated host-specific trimming of mannose residues and possibly, protein trafficking. Notably, the plant-derived viral antigen triggered a significant immune response in vaccinated mice, characterized by the presence of antibodies with HCV neutralizing activity. Together, our study demonstrates that N. benthamiana is a viable alternative to costly mammalian cell cultures for the expression of complex viral antigens and supports the use of plants as cost-effective production platforms for development of HCV vaccines.

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言語: eng - English
 日付: 2021-05
 出版の状態: 出版
 ページ: -
 出版情報: -
 目次: -
 査読: -
 識別子(DOI, ISBNなど): DOI: 10.1111/pbi.13631
 学位: -

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出版物 1

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出版物名: Plant Biotechnology Journal
  その他 : Plant Biotechnol. J.
種別: 学術雑誌
 著者・編者:
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出版社, 出版地: Oxford : Blackwell Pub.
ページ: - 巻号: 19 (10) 通巻号: - 開始・終了ページ: 2027 - 2039 識別子(ISBN, ISSN, DOIなど): ISSN: 1467-7644
CoNE: https://pure.mpg.de/cone/journals/resource/110978984569611