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  The phosphorylated pathway of serine biosynthesis links plant growth with nitrogen metabolism

Zimmermann, S. E., Benstein, R. M., Flores-Tornero, M., Blau, S., Anoman, A. D., Rosa-Téllez, S., et al. (2021). The phosphorylated pathway of serine biosynthesis links plant growth with nitrogen metabolism. Plant Physiology, 186(3), 1487-1506. doi:10.1093/plphys/kiab167.

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Zimmermann, Sandra E1, Author
Benstein, Ruben M1, Author
Flores-Tornero, María1, Author
Blau, Samira1, Author
Anoman, Armand D1, Author
Rosa-Téllez, Sara1, Author
Gerlich, Silke C1, Author
Salem, M.A.2, Author              
Alseekh, S.3, Author              
Kopriva, Stanislav1, Author
Wewer, Vera1, Author
Flügge, Ulf-Ingo1, Author
Jacoby, Richard P1, Author
Fernie, A. R.4, Author              
Giavalisco, P.2, Author              
Ros, Roc1, Author
Krueger, Stephan1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Experimental Systems Biology, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753342              
3The Genetics of Crop Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_3244836              
4Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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 Abstract: Because it is the precursor for various essential cellular components, the amino acid serine is indispensable for every living organism. In plants, serine is synthesized by two major pathways: photorespiration and the phosphorylated pathway of serine biosynthesis (PPSB). However, the importance of these pathways in providing serine for plant development is not fully understood. In this study, we examine the relative contributions of photorespiration and PPSB to providing serine for growth and metabolism in the C3 model plant Arabidopsis thaliana. Our analyses of cell proliferation and elongation reveal that PPSB-derived serine is indispensable for plant growth and its loss cannot be compensated by photorespiratory serine biosynthesis. Using isotope labeling, we show that PPSB-deficiency impairs the synthesis of proteins and purine nucleotides in plants. Furthermore, deficiency in PPSB-mediated serine biosynthesis leads to a strong accumulation of metabolites related to nitrogen metabolism. This result corroborates 15N-isotope labeling in which we observed an increased enrichment in labeled amino acids in PPSB-deficient plants. Expression studies indicate that elevated ammonium uptake and higher GS/GOGAT activity causes this phenotype. Metabolic analyses further show that elevated nitrogen assimilation and reduced amino acid turnover into proteins and nucleotides are the most likely driving forces for changes in respiratory metabolism and amino acid catabolism in PPSB-deficient plants. Accordingly, we conclude that even though photorespiration generates high amounts of serine in plants, PPSB-derived serine is more important for plant growth and its deficiency triggers the induction of nitrogen assimilation, most likely as an amino acid starvation response.

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Language(s): eng - English
 Dates: 2021-06
 Publication Status: Published in print
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 Identifiers: DOI: 10.1093/plphys/kiab167
BibTex Citekey: 10.1093/plphys/kiab167
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 186 (3) Sequence Number: - Start / End Page: 1487 - 1506 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438