English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  High-resolution quantitative profiling of tRNA abundance and modification status in eukaryotes by mim-tRNAseq

Behrens, A., Rodschinka, G., & Nedialkova, D. D. (2021). High-resolution quantitative profiling of tRNA abundance and modification status in eukaryotes by mim-tRNAseq. Molecular Cell, 81(8), 1802-+. doi:10.1016/j.molcel.2021.01.028.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
1-s2.0-S1097276521000484-main.pdf (Publisher version), 6MB
Name:
1-s2.0-S1097276521000484-main.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
Open Access

Locators

show

Creators

show
hide
 Creators:
Behrens, Andrew1, Author              
Rodschinka, Geraldine1, Author              
Nedialkova, Danny D.1, Author              
Affiliations:
1Nedialkova, Danny / Mechanisms of Protein Biogenesis, Max Planck Institute of Biochemistry, Max Planck Society, ou_2466698              

Content

show
hide
Free keywords: MESSENGER-RNA; REVEALS; COMPLEX; SEQ; IDENTIFICATION; MITOCHONDRIAL; RECOGNITION; LANDSCAPE; SEQUENCES; EFFICIENTBiochemistry & Molecular Biology; Cell Biology;
 Abstract: Measurements of cellular tRNA abundance are hampered by pervasive blocks to cDNA synthesis at modified nucleosides and the extensive similarity among tRNA genes. We overcome these limitations with modification-induced misincorporation tRNA sequencing (mim-tRNAseq), which combines a workflow for full-length cDNA library construction from endogenously modified tRNA with a comprehensive and user-friendly computational analysis toolkit. Our method accurately captures tRNA abundance and modification status in yeast, fly, and human cells and is applicable to any organism with a known genome. We applied mim-tRNAseq to discover a dramatic heterogeneity of tRNA isodecoder pools among diverse human cell lines and a surprising interdependence of modifications at distinct sites within the same tRNA transcript.

Details

show
hide
Language(s): eng - English
 Dates: 2021
 Publication Status: Published in print
 Pages: 21
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular Cell
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 81 (8) Sequence Number: - Start / End Page: 1802 - + Identifier: ISSN: 1097-2765
CoNE: https://pure.mpg.de/cone/journals/resource/954925610929