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Free keywords:
ALLERGIC ENCEPHALOMYELITIS; NERVOUS-SYSTEM; CELLS; MACROPHAGES;
EXPRESSION; CD4; INDUCTION; MODEL; MYELIN; RECRUITMENTImmunology; Neurosciences & Neurology; EAE; CFA; CD4(+) T cells; CD8(+) macrophages;
Abstract:
Experimental autoimmune encephalomyelitis (EAE) is classically induced with complete Freund's adjuvant (CFA). The immune response against CFA has a confounding influence on the translational capacity of EAE as a multiple sclerosis model. Here, we compare clinical, cellular and molecular properties between syngeneic spinal cord homogenate (SCH)- and SCH + CFA-immunized Dark Agouti rats. EAE signs were observed earlier and the cumulative clinical score was higher without CFA. Also, a higher number of immune cells infiltrates in the spinal cords was noticed at the peak of EAE without CFA. High spinal cord abundance of CD8(+)CD11bc(+)MHC class II+ cells was detected in SCH-immunized rats. Myelin basic protein -specific response can be elicited in the cells from the lymph nodes draining the site of SCH immunization. This CFA-free EAE is a reliable multiple sclerosis model.