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  Complete Freund's adjuvant-free experimental autoimmune encephalomyelitis in Dark Agouti rats is a valuable tool for multiple sclerosis studies

Lazarevic, M., Djedovic, N., Stanisavljevic, S., Dimitrijevic, M., Stegnjaic, G., Krishnamoorthy, G., et al. (2021). Complete Freund's adjuvant-free experimental autoimmune encephalomyelitis in Dark Agouti rats is a valuable tool for multiple sclerosis studies. Journal of Neuroimmunology, 354: 577547. doi:10.1016/j.jneuroim.2021.577547.

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 Creators:
Lazarevic, Milica1, Author
Djedovic, Neda1, Author
Stanisavljevic, Suzana1, Author
Dimitrijevic, Mirjana1, Author
Stegnjaic, Goran1, Author
Krishnamoorthy, Gurumoorthy2, Author           
Stojkovic, Marija Mostarica1, Author
Miljkovic, Dorde1, Author
Jevtic, Bojan1, Author
Affiliations:
1external, ou_persistent22              
2Krishnamoorthy, Gurumoorthy / Neuroinflammation and Mucosal Immunology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2173635              

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Free keywords: ALLERGIC ENCEPHALOMYELITIS; NERVOUS-SYSTEM; CELLS; MACROPHAGES; EXPRESSION; CD4; INDUCTION; MODEL; MYELIN; RECRUITMENTImmunology; Neurosciences & Neurology; EAE; CFA; CD4(+) T cells; CD8(+) macrophages;
 Abstract: Experimental autoimmune encephalomyelitis (EAE) is classically induced with complete Freund's adjuvant (CFA). The immune response against CFA has a confounding influence on the translational capacity of EAE as a multiple sclerosis model. Here, we compare clinical, cellular and molecular properties between syngeneic spinal cord homogenate (SCH)- and SCH + CFA-immunized Dark Agouti rats. EAE signs were observed earlier and the cumulative clinical score was higher without CFA. Also, a higher number of immune cells infiltrates in the spinal cords was noticed at the peak of EAE without CFA. High spinal cord abundance of CD8(+)CD11bc(+)MHC class II+ cells was detected in SCH-immunized rats. Myelin basic protein -specific response can be elicited in the cells from the lymph nodes draining the site of SCH immunization. This CFA-free EAE is a reliable multiple sclerosis model.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Journal of Neuroimmunology
  Other : J. Neuroimmunol.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 354 Sequence Number: 577547 Start / End Page: - Identifier: ISSN: 0165-5728
CoNE: https://pure.mpg.de/cone/journals/resource/954925481617