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  Microscale Thermophoresis and additional effects measured in NanoTemper Monolith instruments

Lopez-Mendez, B., Uebel, S., Lundgren, L. P., & Sedivy, A. (2021). Microscale Thermophoresis and additional effects measured in NanoTemper Monolith instruments. European Biophysics Journal with Biophysics Letters. doi:10.1007/s00249-021-01529-1.

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 Creators:
Lopez-Mendez, Blanca1, Author
Uebel, Stephan2, Author              
Lundgren, Li Peng1, Author
Sedivy, Arthur1, Author
Affiliations:
1external, ou_persistent22              
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: Biophysics; MST; TRIC; Thermophoresis; K-D; Interaction;
 Abstract: NanoTemper Monolith instruments have gained enormous popularity for measuring molecular interactions both in academia and industry. The underlying technology has been extensively reviewed along with its assumptions, limitations, and applications (Scheuermann et al., Anal Biochem 496:79-93, 2016). Several assumptions about the technique such as the extent of thermal deviations generated by the infrared laser and the thermophoretic foundation of the measured signal have been revised during the last decade. We present here in this letter the experience gathered in scientific service facilities about this technique and make scientists aware of possible pitfalls with the intention to promote knowledge and good practice throughout the scientific community.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: European Biophysics Journal with Biophysics Letters
  Other : European Biophysics Journal with Biophysics Letters
Source Genre: Journal
 Creator(s):
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Publ. Info: Berlin : Springer
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0175-7571
CoNE: https://pure.mpg.de/cone/journals/resource/954925487773