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  Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2

Fianu, I., Dienemann, C., Aibara, S., Schilbach, S., & Cramer, P. (2021). Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2. Communications Biology, 4: 606. doi:10.1038/s42003-021-02088-z.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-A650-2 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-A65C-6
Genre: Journal Article

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 Creators:
Fianu, I.1, Author           
Dienemann, C.1, Author           
Aibara, S.1, Author           
Schilbach, S.1, Author           
Cramer, P.1, Author           
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1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: Cryoelectron microscopy; Transcription
 Abstract: Nuclear import of RNA polymerase II (Pol II) involves the conserved factor RPAP2. Here we report the cryo-electron microscopy (cryo-EM) structure of mammalian Pol II in complex with human RPAP2 at 2.8 Å resolution. The structure shows that RPAP2 binds between the jaw domains of the polymerase subunits RPB1 and RPB5. RPAP2 is incompatible with binding of downstream DNA during transcription and is displaced upon formation of a transcription pre-initiation complex.

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Language(s): eng - English
 Dates: 2021-05-21
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s42003-021-02088-z
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Title: Communications Biology
Source Genre: Journal
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Pages: 7 Volume / Issue: 4 Sequence Number: 606 Start / End Page: - Identifier: -