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  Giant MDCK cells: A novel expression system

Oberleithner, H., Schwab, A., Westphale, H. J., Schuricht, B., Püschel, B., & Koepsell, H. (1990). Giant MDCK cells: A novel expression system. In S. Fleischer, & B. Fleischer (Eds.), Methods in Enzymology (pp. 437-449). Academic Press; Elsevier Inc. doi:10.1016/0076-6879(90)91028-5.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-A6B3-2 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-A6B4-1
Genre: Book Chapter

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 Creators:
Oberleithner, Hans1, Author
Schwab, Albrecht1, Author
Westphale, Hans Jürgen1, Author
Schuricht, Barbara1, Author
Püschel, Bernd2, Author           
Koepsell, Hermann2, Author           
Affiliations:
1Julius-Maximilians-Universität Würzburg, Würzburg, Germany, ou_persistent22              
2Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              

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 Abstract: This chapter describes a technique by which an epithelial cell line can be used for expression experiments. Basically, cells are harvested from a subconfluent monolayer and fused to form giant cells. For expression experiments the giant cells have to be identified first, then injected individually, and, finally, 24 hr after microinjection, re identified to perform the electrical analysis. After injection the cells are documented in terms of their location and the amount of injected mRNA so that the injected cells can be identified individually after 24 hr. After injection the Ringer solution is exchanged by culture medium (plus antibiotics) and the injected cells are incubated for expression. About 24 hr after mRNA injection the injected cells are studied by electrophysiological techniques. Intracellular cell membrane and specific plasma membrane resistances of fused Madin-Darby Canine Kidney Cells (MDCK) cells are measured according to techniques described in this chapter.

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Language(s): eng - English
 Dates: 2004-01-071990
 Publication Status: Issued
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0076-6879(90)91028-5
 Degree: -

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Title: Methods in Enzymology
  Subtitle : Biomembranes Part V: Cellular and Subcellular Transport: Epithelial Cells
Source Genre: Series
 Creator(s):
Fleischer, Sidney, Editor
Fleischer, Becca, Editor
Affiliations:
-
Publ. Info: Academic Press; Elsevier Inc
Pages: 939 Volume / Issue: 191 Sequence Number: - Start / End Page: 437 - 449 Identifier: ISBN: 978-0-12-182093-0
ISSN: 0076-6879