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  Podosome-Driven Defect Development in Lamellar Bone under the Conditions of Senile Osteoporosis Observed at the Nanometer Scale

Simon, P., Pompe, W., Bobeth, M., Worch, H., Kniep, R., Formanek, P., et al. (2021). Podosome-Driven Defect Development in Lamellar Bone under the Conditions of Senile Osteoporosis Observed at the Nanometer Scale. ACS Biomaterials Science & Engineering, 7(6), 2255-2267. doi:10.1021/acsbiomaterials.0c01493.

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 Creators:
Simon, Paul1, Author              
Pompe, Wolfgang2, Author
Bobeth, Manfred2, Author
Worch, Hartmut2, Author
Kniep, Rüdiger3, Author              
Formanek, Petr2, Author
Hild, Anne2, Author
Wenisch, Sabine2, Author
Sturm, Elena4, Author              
Affiliations:
1Paul Simon, Chemical Metal Science, Max Planck Institute for Chemical Physics of Solids, Max Planck Society, ou_1863418              
2External Organizations, ou_persistent22              
3Rüdiger Kniep, Inorganic Chemistry, Max Planck Institute for Chemical Physics of Solids, Max Planck Society, ou_1863437              
4Max Planck Institute for Chemical Physics of Solids, Max Planck Society, ou_1863404              

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Free keywords: bone, femur, human, podosome, SEM, senile osteoporosis, TEM, trabecula, ultrastructure, Collagen, Degradation, Diseases, Disintegration, High resolution electron microscopy, Hydroxyapatite, Microcracks, Phosphate minerals, Plates (structural components), Bone metabolism, Collagen fibrils, Degradation mechanism, Extracellular bone matrices, Human trabecular bone, Mineralized collagen fibrils, Nano-meter scale, Plywood structures, Bone
 Abstract: The degradation mechanism of human trabecular bone harvested from the central part of the femoral head of a patient with a fragility fracture of the femoral neck under conditions of senile osteoporosis was investigated by high-resolution electron microscopy. As evidenced by light microscopy, there is a disturbance of bone metabolism leading to severe and irreparable damages to the bone structure. These defects are evoked by osteoclasts and thus podosome activity. Podosomes create typical pit marks and holes of about 300-400 nm in diameter on the bone surface. Detailed analysis of the stress field caused by the podosomes in the extracellular bone matrix was performed. The calculations yielded maximum stress in the range of few megapascals resulting in formation of microcracks around the podosomes. Disintegration of hydroxyapatite and free lying collagen fibrils were observed at the edges of the plywood structure of the bone lamella. At the ultimate state, the disintegration of the mineralized collagen fibrils to a gelatinous matrix comes along with a delamination of the apatite nanoplatelets resulting in a brittle, porous bone structure. The nanoplatelets aggregate to big hydroxyapatite plates with a size of up to 10 x 20 μm2. The enhanced plate growth can be explained by the interaction of two mechanisms in the ruffled border zone: the accumulation of delaminated hydroxyapatite nanoplatelets near clusters of podosomes and the accelerated nucleation and random growth of HAP nanoplatelets due to a nonsufficient concentration of process-directing carboxylated osteocalcin cOC. © 2021 The Authors. Published by American Chemical Society.

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Language(s): eng - English
 Dates: 2021-05-032021-05-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1021/acsbiomaterials.0c01493
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Title: ACS Biomaterials Science & Engineering
  Abbreviation : acs biomater. sci. eng.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 7 (6) Sequence Number: - Start / End Page: 2255 - 2267 Identifier: ISSN: 2373-9878
CoNE: https://pure.mpg.de/cone/journals/resource/2373-9878