English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Synaptotagmin-1 interacts with PI(4,5)P2 to initiate synaptic vesicle docking in hippocampal neurons

Chen, Y., Wang, Y.-H., Zheng, Y., Li, M., Wang, B., Wang, Q.-W., et al. (2021). Synaptotagmin-1 interacts with PI(4,5)P2 to initiate synaptic vesicle docking in hippocampal neurons. Cell Reports, 34(11): 108842. doi:10.1016/j.celrep.2021.108842.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
1-s2.0-S221112472100156X-main.pdf (Publisher version), 7MB
Name:
1-s2.0-S221112472100156X-main.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
open access
:
ScienceDirect_files_09Nov2021_10-36-37.804.zip (Supplementary material), 15MB
Name:
ScienceDirect_files_09Nov2021_10-36-37.804.zip
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/zip / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Chen, Yun1, Author
Wang, Ying-Han1, Author
Zheng, Yi1, Author
Li, Meijing2, Author              
Wang, Bing1, Author
Wang, Qiu-Wen1, Author
Fu, Chong-Lei1, Author
Liu, Yao-Nan1, Author
Li, Xueming1, Author
Yao, Jun1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

Content

show
hide
Free keywords: MEMBRANE; SYNTAXIN; MUTANTS; RELEASE; PIP2; RECRUITMENT; METABOLISM; ACTIVATION; PATHWAY; BINDINGCell Biology;
 Abstract: Synaptic vesicle (SV) docking is a dynamic multi-stage process that is required for efficient neurotransmitter release in response to nerve impulses. Although the steady-state SV docking likely involves the cooperation of Synaptotagmin-1 (Syt1) and soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), where and how the docking process initiates remains unknown. Phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) can interact with Syt1 and SNAREs to contribute to vesicle exocytosis. In the present study, using the CRISPRi-mediated multiplex gene knockdown and 3D electron tomography approaches, we show that in mouse hippocampal synapses, SV docking initiates at similar to 12 nm to the active zone (AZ) by Syt1. Furthermore, we demonstrate that PI(4,5)P2 is the membrane partner of Syt1 to initiate SV docking, and disrupting their interaction could abolish the docking initiation. In contrast, the SNARE complex contributes only to the tight SV docking within 0-2 nm. Therefore, Syt1 interacts with PI(4,5)P2 to loosely dock SVs within 2-12 nm to the AZ in hippocampal neurons.

Details

show
hide
Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 21
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cell Reports
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Maryland Heights, MO : Cell Press
Pages: - Volume / Issue: 34 (11) Sequence Number: 108842 Start / End Page: - Identifier: ISSN: 2211-1247
CoNE: https://pure.mpg.de/cone/journals/resource/2211-1247