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  Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Protein Vesicle Tracking in HeLa Cells

Pacheco-Fernandez, N., Pakdel, M., & von Blume, J. (2021). Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Protein Vesicle Tracking in HeLa Cells. Bio-protocol, 11(5): e3936. doi:10.21769/BioProtoc.3936.

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Genre: Journal Article
Other : protocol /open access

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Locator:
https://pubmed.ncbi.nlm.nih.gov/32479594/ (Supplementary material)
Description:
original research article

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 Creators:
Pacheco-Fernandez, Natalia1, Author              
Pakdel, Mehrshad1, Author              
von Blume, Julia2, Author
Affiliations:
1Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              
2external, ou_persistent22              

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Free keywords: Life Sciences & Biomedicine - Other Topics; Protein trafficking; RUSH; Cargo sorting; Confocal microscopy; Vesicle tracking;
 Abstract: Monitoring vesicle trafficking is an excellent tool for the evaluation of protein dynamics in living cells. Such study is key for the understanding of protein sorting and secretion. Recent developments in microscopy, as well as new methodologies developed to study synchronized trafficking of proteins, allowed a better understanding of signaling, regulation and trafficking dynamics at the secretory pathway. One of the most helpful tools so far developed is the Retention Using Selective Hooks (RUSH) system, a methodology that facilitates the evaluation of synchronized cargo trafficking by monitoring fluorescent vesicles in cells upon biotin addition. Here we present a protocol that allows the quantitative evaluation of protein cargo trafficking at different fixed time points and an analytic approach that enables a better examination of specific cargo trafficking dynamics at the secretory pathway. [GRAPHICS]

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000627055300006
DOI: 10.21769/BioProtoc.3936
 Degree: -

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Title: Bio-protocol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Berkley : Bio Online
Pages: - Volume / Issue: 11 (5) Sequence Number: e3936 Start / End Page: - Identifier: ISSN: 2331-8325
CoNE: https://pure.mpg.de/cone/journals/resource/2331-8325