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  Molecular classification of zebrafish retinal ganglion cells links genes to cell types to behavior

Koelsch, Y., Hahn, J., Sappington, A., Stemmer, M., Fernandes, A. M., Helmbrecht, T. O., et al. (2021). Molecular classification of zebrafish retinal ganglion cells links genes to cell types to behavior. Neuron, 109(4), 645-662.e9. doi:10.1016/j.neuron.2020.12.003.

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 Creators:
Koelsch, Yvonne1, Author           
Hahn, Joshua, Author
Sappington, Anna, Author
Stemmer, Manuel1, Author           
Fernandes, António M.1, Author           
Helmbrecht, Thomas O.1, Author           
Lele, Shriya1, Author           
Butrus, Salwan, Author
Laurell, Eva1, Author           
Arnold-Ammer, Irene1, Author           
Shekhar, Karthik, Author
Sanes, Joshua R., Author
Baier, Herwig1, Author           
Affiliations:
1Department: Genes-Circuits-Behavior / Baier, MPI of Neurobiology, Max Planck Society, ou_1128545              

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Free keywords: Neurosciences & Neurology;
 Abstract: Retinal ganglion cells (RGCs) form an array of feature detectors, which convey visual information to central brain regions. Characterizing RGC diversity is required to understand the logic of the underlying functional segregation. Using single-cell transcriptomics, we systematically classified RGCs in adult and larval zebra fish, thereby identifying marker genes for >30 mature types and several developmental intermediates. We used this dataset to engineer transgenic driver lines, enabling specific experimental access to a subset of RGC types. Expression of one or few transcription factors often predicts dendrite morphologies and axonal projections to specific tectal layers and extratectal targets. In vivo calcium imaging revealed that molecularly defined RGCs exhibit specific functional tuning. Finally, chemogenetic ablation of eomesa+ RGCs, which comprise melanopsin-expressing types with projections to a small subset of central targets, selectively impaired phototaxis. Together, our study establishes a framework for systematically studying the functional architecture of the visual system.

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Language(s): eng - English
 Dates: 2021-02-17
 Publication Status: Published in print
 Pages: 28
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Neuron
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 109 (4) Sequence Number: - Start / End Page: 645 - 662.e9 Identifier: ISSN: 0896-6273
CoNE: https://pure.mpg.de/cone/journals/resource/954925560565