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  Trans-synaptic assemblies link synaptic vesicles and neuroreceptors

Martinez Sanchez, A., Laugks, U., Kochovski, Z., Papantoniou, C., Zinzula, L., Baumeister, W., et al. (2021). Trans-synaptic assemblies link synaptic vesicles and neuroreceptors. Science Advances, 7(10): eabe6204. doi:10.1126/sciadv.abe6204.

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Genre: Journal Article

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 Creators:
Martinez Sanchez, Antonio1, Author           
Laugks, Ulrike1, Author           
Kochovski, Zdravko1, Author           
Papantoniou, Christos1, Author           
Zinzula, Luca1, Author           
Baumeister, Wolfgang1, Author           
Lucic, Vladan1, Author           
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: PERCOLL GRADIENT PROCEDURE; AMPA RECEPTOR; POSTSYNAPTIC DENSITY; CRYOELECTRON TOMOGRAPHY; ARCHITECTURE; SYNAPSES; ORGANIZATION; CLEFT; VISUALIZATION; LOCALIZATIONScience & Technology - Other Topics;
 Abstract: Synaptic transmission is characterized by fast, tightly coupled processes and complex signaling pathways that require a precise protein organization, such as the previously reported nanodomain colocalization of pre- and postsynaptic proteins. Here, we used cryo-electron tomography to visualize synaptic complexes together with their native environment comprising interacting proteins and lipids on a 2- to 4-nm scale. Using template-free detection and classification, we showed that tripartite trans-synaptic assemblies (subcolumns) link synaptic vesicles to postsynaptic receptors and established that a particular displacement between directly interacting complexes characterizes subcolumns. Furthermore, we obtained de novo average structures of ionotropic glutamate receptors in their physiological composition, embedded in plasma membrane. These data support the hypothesis that synaptic function is carried by precisely organized trans-synaptic units. It provides a framework for further exploration of synaptic and other large molecular assemblies that link different cells or cellular regions and may require weak or transient interactions to exert their function.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000628612000010
DOI: 10.1126/sciadv.abe6204
 Degree: -

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Title: Science Advances
  Other : Sci. Adv.
Source Genre: Journal
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Publ. Info: Washington : AAAS
Pages: - Volume / Issue: 7 (10) Sequence Number: eabe6204 Start / End Page: - Identifier: ISSN: 2375-2548
CoNE: https://pure.mpg.de/cone/journals/resource/2375-2548