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  The Novel Halovirus Hardycor1, and the Presence of Active (Induced) Proviruses in Four Haloarchaea

Dyall-Smith, M., Pfeiffer, F., Chiang, P.-W., & Tang, S.-L. (2021). The Novel Halovirus Hardycor1, and the Presence of Active (Induced) Proviruses in Four Haloarchaea. Genes, 12(2): 149. doi:10.3390/genes12020149.

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Genre: Journal Article

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 Creators:
Dyall-Smith, Mike1, Author              
Pfeiffer, Friedhelm1, Author              
Chiang, Pei-Wen2, Author
Tang, Sen-Lin2, Author
Affiliations:
1Habermann, Bianca / Computational Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1832284              
2external, ou_persistent22              

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Free keywords: Genetics & Heredity; archaea; haloarchaea; temperate virus; Haloferax; Haloarcula; Halorubrum; halobacteria; pleolipovirus; caudovirus; siphovirus;
 Abstract: The virus Hardycor1 was isolated in 1998 and infects the haloarchaeon Halorubrum coriense. DNA from a frozen stock (HC1) was sequenced and the viral genome found to be 45,142 bp of dsDNA, probably having redundant, circularly permuted termini. The genome showed little similarity (BLASTn) to known viruses. Only twenty-two of the 53 (41%) predicted proteins were significantly similar to sequences in the NCBI nr protein database (E-value <= 10(-15)). Six caudovirus-like proteins were encoded, including large subunit terminase (TerL), major capsid protein (Mcp) and tape measure protein (Tmp). Hardycor1 was predicted to be a siphovirus (VIRFAM). No close relationship to other viruses was found using phylogenetic tree reconstructions based on TerL and Mcp. Unexpectedly, the sequenced virus stock HC1 also revealed two induced proviruses of the host: a siphovirus (Humcor1) and a pleolipovirus (Humcor2). A re-examination of other similarly sequenced, archival virus stocks revealed induced proviruses of Haloferax volcanii, Haloferax gibbonsii and Haloarcula hispanica, three of which were pleolipoviruses. One provirus (Halfvol2) of Hfx. volcanii showed little similarity (BLASTn) to known viruses and probably represents a novel virus group. The attP sequences of many pleolipoproviruses were found to be embedded in a newly detected coding sequence, split in the provirus state, that spans between genes for integrase and a downstream CxxC-motif protein. This gene might play an important role in regulation of the temperate state.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 23
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000622586300001
DOI: 10.3390/genes12020149
 Degree: -

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Title: Genes
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI AG
Pages: - Volume / Issue: 12 (2) Sequence Number: 149 Start / End Page: - Identifier: ISSN: 2073-4425
CoNE: https://pure.mpg.de/cone/journals/resource/2073-4425