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  Human NLRP1 is a sensor for double-stranded RNA

Bauernfried, S., Scherr, M. J., Pichlmair, A., Duderstadt, K. E., & Hornung, V. (2021). Human NLRP1 is a sensor for double-stranded RNA. Science, 371(6528): eabd0811, pp. 482-482. doi:10.1126/science.abd0811.

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Genre: Journal Article

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 Creators:
Bauernfried, Stefan1, Author
Scherr, Matthias J.2, Author              
Pichlmair, Andreas1, Author
Duderstadt, Karl E.2, Author              
Hornung, Veit3, Author              
Affiliations:
1external, ou_persistent22              
2Duderstadt, Karl / Structure and Dynamics of Molecular Machines, Max Planck Institute of Biochemistry, Max Planck Society, ou_2265639              
3Hornung, Veit / Molecular Mechanisms of Inflammation, Max Planck Institute of Biochemistry, Max Planck Society, ou_3185245              

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Free keywords: ANTIVIRAL COMPOUNDS; CELL-DEATH; INFLAMMASOME; MECHANISM; RECOGNITION; INHIBITION; ACTIVATION; REVEALSScience & Technology - Other Topics;
 Abstract: Inflammasomes function as intracellular sensors of pathogen infection or cellular perturbation and thereby play a central role in numerous diseases. Given the high abundance of NLRP1 in epithelial barrier tissues, we screened a diverse panel of viruses for inflammasome activation in keratinocytes. We identified Semliki Forest virus (SFV), a positive-strand RNA virus, as a potent activator of human but not murine NLRP1B. SFV replication and the associated formation of double-stranded (ds) RNA was required to engage the NLRP1 inflammasome. Moreover, delivery of long dsRNA was sufficient to trigger activation. Biochemical studies revealed that NLRP1 binds dsRNA through its leucine-rich repeat domain, resulting in its NACHT domain gaining adenosine triphosphatase activity. Altogether, these results establish human NLRP1 as a direct sensor for dsRNA and thus RNA virus infection.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000612983100035
DOI: 10.1126/science.abd0811
 Degree: -

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Project name : ERC-2014-CoG–647858 GENESIS
Grant ID : 647858
Funding program : -
Funding organization : European Research Council

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Title: Science
  Abbreviation : Science
Source Genre: Journal
 Creator(s):
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 371 (6528) Sequence Number: eabd0811 Start / End Page: 482 - 482 Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1