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  Presence of luminal K+, a prerequisite for active NaCl transport in the cortical thick ascending limb of Henle's loop of rabbit kidney

Greger, R., & Schlatter, E. (1981). Presence of luminal K+, a prerequisite for active NaCl transport in the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Archiv: European Journal of Physiology, 392(1), 92-94. doi:10.1007/BF00584588.

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 Creators:
Greger, Rainer1, Author           
Schlatter, Eberhard1, Author           
Affiliations:
1Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              

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Free keywords: Isolated perfused tubule; Rabbit cortical thick ascending limb of Henle's loop; Sodium potassium-chloride cotransport; Ba2+
 Abstract: Previous data from our laboratory have shown that active transport in the cortical thick ascending limb of Henle's loop (cTAL), as measured by the short circuit current (Lsc, microA X cm−2), requires the presence of Na+ and Cl. The data were compatible with the model of secondarily active Cl reabsorption involving the cotransport of Na+ and Cl across the luminal membrane. The data suggested, furthermore, that 1 Na+ and 2 Cl interact with the luminal carrier. In the present study it was tested whether this reabsorptive mechanism also requires the presence of luminal K+. Isolated cTAL segments (n = 40) were perfused at high flow rates with a modified Ringer's solution. Removal of K+ from the lumen reduced Isc significantly from 215 to 133 microA X cm−2. Addition of Ba2+ (10−3 mol X 1−1) which blocks the K+ conductance of the luminal, membrane, to the K+-containing lumen perfusate decreased Isc significantly from 234 to 141 microA X cm−2. Combination of both manoeuvres: perfusion with a K+-free and Ba2+-containing solution almost abolished Isc from a control of 237 to 56 microA X cm−2. The results are compatible with the view that in rabbit cTAL the luminal carrier interacts with all 3 ions, possibly 1 Na+, 2 Cl, and 1 K+. K+ recycles across the luminal membrane through its conductive pathway.

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Language(s): eng - English
 Dates: 1981-09-231981-09-281981-11-01
 Publication Status: Issued
 Pages: 3
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/BF00584588
PMID: 7322839
 Degree: -

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Title: Pflügers Archiv: European Journal of Physiology
  Other : Pflügers Arch. Europ. J. Physiol.
Source Genre: Journal
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Publ. Info: Heidelberg : Springer-Verlag
Pages: - Volume / Issue: 392 (1) Sequence Number: - Start / End Page: 92 - 94 Identifier: ISSN: 0031-6768
CoNE: https://pure.mpg.de/cone/journals/resource/954925432380