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  Analysis of Ca2+ fluxes and Ca2+ pools in pancreatic acini

Schulz, I., Kimura, T., Wakasugi, H., Haase, W., & Kribben, A. (1981). Analysis of Ca2+ fluxes and Ca2+ pools in pancreatic acini. Philosophical Transactions of the Royal Society of London, Series B: Biological Sciences, 296(1080), 105-113. doi:10.1098/rstb.1981.0175.

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 Creators:
Schulz, Irene1, Author           
Kimura, Toshinari1, Author           
Wakasugi, Hideyuki1, Author           
Haase, Winfried1, Author           
Kribben, Andreas1, Author           
Affiliations:
1Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              

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 Abstract: 45Ca2+ movements have been analysed in dispersed acini prepared from rat pancreas in a quasi-steady state for 45Ca2+. Carbamyl choline (carbachol; Cch) caused a quick 45Ca2+ release that was followed by a slower 45Ca2+ ‘reuptake’. Subsequent addition of atropine resulted in a further transient increase in cellular 45Ca2+. The data suggest the presence of a Cch-sensitive ‘trigger’ pool, which could be refilled by the antagonist, and one or more intracellular ‘storage’ pools. Intracellular Ca2+ sequestration was studied in isolated acini pretreated with saponin to disrupt their plasma membranes. In the presence of 45Ca2+ (1 µM), addition of ATP at 5 mM caused a rapid increase in 45Ca2+ uptake exceeding the control by fivefold. Maximal ATP-promoted Ca2+ uptake was obtained at 10 µM Ca2+ (half-maximal at 0.32 µM Ca2+). In the presence of mitochondrial inhibitors it was 0.1 µM (half-maximal at 0.014 µM). 45Ca2+ release could still be induced by Cch but the subsequent reuptake was missing. The latter was restored by ATP and atropine caused further 45Ca2+ uptake. Electron microscopy showed electron-dense precipitates in the rough endoplasmic reticulum of saponin-treated cells in the presence of Ca2+, oxalate and ATP which were absent in intact cells or cells pretreated with A23187. The data suggest the presence of a plasma membrane-bound Cch-sensitive ‘trigger’ Ca2+ pool and ATP-dependent Ca2+ storage systems in mitochondria and rough endoplasmic reticulum of pancreatic acini. It is assumed that Ca2+ is taken up into these pools after secretagogue-induced Ca2+ release.

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Language(s): eng - English
 Dates: 1981-07-141997-01-011981-12-18
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1098/rstb.1981.0175
PMID: 6121336
 Degree: -

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Title: Philosophical Transactions of the Royal Society of London, Series B: Biological Sciences
  Other : Philosophical Transactions B
  Abbreviation : Phil. Trans. R. Soc. B
Source Genre: Journal
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Publ. Info: London : Royal Society
Pages: - Volume / Issue: 296 (1080) Sequence Number: - Start / End Page: 105 - 113 Identifier: ISSN: 0962-8436
CoNE: https://pure.mpg.de/cone/journals/resource/963017382021_1