English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Cultivation of halophilic archaea (class Halobacteria) from thalassohaline and athalassohaline environments

Cui, H.-L., & Dyall-Smith, M. L. (2021). Cultivation of halophilic archaea (class Halobacteria) from thalassohaline and athalassohaline environments. Marine Life Science & Technology, 3(2), 243-251. doi:10.1007/s42995-020-00087-3.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Cui, Heng-Lin1, Author
Dyall-Smith, Mike L.2, Author              
Affiliations:
1external, ou_persistent22              
2Habermann, Bianca / Computational Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1832284              

Content

show
hide
Free keywords: DEAD-SEA HALOBACTERIUM; IN-SITU CULTIVATION; SP NOV.; GEN. NOV.; SP. NOV.; ORDER HALOBACTERIALES; EMENDED DESCRIPTION; DEEP LAKE; RED-SEA; HALOARCHAEONHalobacteria; Growth; Culture; Cultivation; Large-scale isolation; Medium formulation;
 Abstract: As a group, the halophilic archaea (class Halobacteria) are the most salt-requiring and salt-resistant microorganisms within the domain Archaea. Halophilic archaea flourish in thalassohaline and athalassohaline environments and require over 100-150 g/L NaCl for growth and structural stability. Natural hypersaline environments vary in salt concentration, chemical composition and pH, and occur in climates ranging from tropical to polar and even under-sea. Accordingly, their resident haloarchaeal species vary enormously, as do their individual population compositions and community structures. These diverse halophilic archaeal strains are precious resources for theoretical and applied research but assessing their taxonomic and metabolic novelty and diversity in natural environments has been technically difficult up until recently. Environmental DNA-based high-throughput sequencing technology has now matured sufficiently to allow inexpensive recovery of massive amounts of sequence data, revealing the distribution and community composition of halophilic archaea in different hypersaline environments. While cultivation of haloarchaea is slow and tedious, and only recovers a fraction of the natural diversity, it is the conventional means of describing new species, and provides strains for detailed study. As of the end of May 2020, the class Halobacteria contains 71 genera and 275 species, 49.8% of which were first isolated from the marine salt environment and 50.2% from the inland salt environment, indicating that both thalassohaline and athalassohaline environments contain diverse halophilic archaea. However, there remain taxa that have not yet been isolated in pure culture, such as the nanohaloarchaea, which are widespread in the salt environment and may be one of the hot spots in the field of halophilic archaea research in the future. In this review, we focus on the cultivation strategies that have been used to isolate extremely halophilic archaea and point out some of the pitfalls and challenges.

Details

show
hide
Language(s): eng - English
 Dates: 2021
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Marine Life Science & Technology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: CAMPUS, 4 CRINAN ST, LONDON, N1 9XW, ENGLAND : SPRINGERNATURE
Pages: - Volume / Issue: 3 (2) Sequence Number: - Start / End Page: 243 - 251 Identifier: ISSN: 2096-6490

Source 2

show
hide
Title: Marine Life Science & Technology
  Alternative Title : MAR LIFE SCI TECH
  Alternative Title : Mar. Life Sci. Tech.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 3 Sequence Number: - Start / End Page: - Identifier: -