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  Metabolite Profiling in Arabidopsisthaliana with Moderately Impaired Photorespiration Reveals Novel Metabolic Links and Compensatory Mechanisms of Photorespiration

Timm, S., Nunes-Nesi, A., Florian, A., Eisenhut, M., Morgenthal, K., Wirtz, M., et al. (2021). Metabolite Profiling in Arabidopsisthaliana with Moderately Impaired Photorespiration Reveals Novel Metabolic Links and Compensatory Mechanisms of Photorespiration. Metabolites, 11(6): 391. doi:10.3390/metabo11060391.

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Timm, Stefan1, Author
Nunes-Nesi, A.2, Author              
Florian, A.2, Author              
Eisenhut, Marion1, Author
Morgenthal, K.3, Author              
Wirtz, Markus1, Author
Hell, Rüdiger1, Author
Weckwerth, W.3, Author              
Hagemann, Martin1, Author
Fernie, A. R.2, Author              
Bauwe, Hermann1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
3Integrative Proteomics and Metabolomics, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753334              

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 Abstract: Photorespiration is an integral component of plant primary metabolism. Accordingly, it has been often observed that impairing the photorespiratory flux negatively impacts other cellular processes. In this study, the metabolic acclimation of the Arabidopsisthaliana wild type was compared with the hydroxypyruvate reductase 1 (HPR1; hpr1) mutant, displaying only a moderately reduced photorespiratory flux. Plants were analyzed during development and under varying photoperiods with a combination of non-targeted and targeted metabolome analysis, as well as 13C- and 14C-labeling approaches. The results showed that HPR1 deficiency is more critical for photorespiration during the vegetative compared to the regenerative growth phase. A shorter photoperiod seems to slowdown the photorespiratory metabolite conversion mostly at the glycerate kinase and glycine decarboxylase steps compared to long days. It is demonstrated that even a moderate impairment of photorespiration severely reduces the leaf-carbohydrate status and impacts on sulfur metabolism. Isotope labeling approaches revealed an increased CO2 release from hpr1 leaves, most likely occurring from enhanced non-enzymatic 3-hydroxypyruvate decarboxylation and a higher flux from serine towards ethanolamine through serine decarboxylase. Collectively, the study provides evidence that the moderate hpr1 mutant is an excellent tool to unravel the underlying mechanisms governing the regulation of metabolic linkages of photorespiration with plant primary metabolism.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: DOI: 10.3390/metabo11060391
BibTex Citekey: metabo11060391
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Title: Metabolites
Source Genre: Journal
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Publ. Info: MDPI AG
Pages: - Volume / Issue: 11 (6) Sequence Number: 391 Start / End Page: - Identifier: Other: 2218-1989
CoNE: https://pure.mpg.de/cone/journals/resource/2218-1989