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  Metabolite Profiling in Arabidopsisthaliana with Moderately Impaired Photorespiration Reveals Novel Metabolic Links and Compensatory Mechanisms of Photorespiration

Timm, S., Nunes-Nesi, A., Florian, A., Eisenhut, M., Morgenthal, K., Wirtz, M., et al. (2021). Metabolite Profiling in Arabidopsisthaliana with Moderately Impaired Photorespiration Reveals Novel Metabolic Links and Compensatory Mechanisms of Photorespiration. Metabolites, 11(6): 391. doi:10.3390/metabo11060391.

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Timm, Stefan1, Autor
Nunes-Nesi, A.2, Autor           
Florian, A.2, Autor           
Eisenhut, Marion1, Autor
Morgenthal, K.3, Autor           
Wirtz, Markus1, Autor
Hell, Rüdiger1, Autor
Weckwerth, W.3, Autor           
Hagemann, Martin1, Autor
Fernie, A. R.2, Autor           
Bauwe, Hermann1, Autor
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
3Integrative Proteomics and Metabolomics, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753334              

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 Zusammenfassung: Photorespiration is an integral component of plant primary metabolism. Accordingly, it has been often observed that impairing the photorespiratory flux negatively impacts other cellular processes. In this study, the metabolic acclimation of the Arabidopsisthaliana wild type was compared with the hydroxypyruvate reductase 1 (HPR1; hpr1) mutant, displaying only a moderately reduced photorespiratory flux. Plants were analyzed during development and under varying photoperiods with a combination of non-targeted and targeted metabolome analysis, as well as 13C- and 14C-labeling approaches. The results showed that HPR1 deficiency is more critical for photorespiration during the vegetative compared to the regenerative growth phase. A shorter photoperiod seems to slowdown the photorespiratory metabolite conversion mostly at the glycerate kinase and glycine decarboxylase steps compared to long days. It is demonstrated that even a moderate impairment of photorespiration severely reduces the leaf-carbohydrate status and impacts on sulfur metabolism. Isotope labeling approaches revealed an increased CO2 release from hpr1 leaves, most likely occurring from enhanced non-enzymatic 3-hydroxypyruvate decarboxylation and a higher flux from serine towards ethanolamine through serine decarboxylase. Collectively, the study provides evidence that the moderate hpr1 mutant is an excellent tool to unravel the underlying mechanisms governing the regulation of metabolic linkages of photorespiration with plant primary metabolism.

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Sprache(n): eng - English
 Datum: 2021
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
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 Identifikatoren: DOI: 10.3390/metabo11060391
BibTex Citekey: metabo11060391
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Titel: Metabolites
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: MDPI AG
Seiten: - Band / Heft: 11 (6) Artikelnummer: 391 Start- / Endseite: - Identifikator: Anderer: 2218-1989
CoNE: https://pure.mpg.de/cone/journals/resource/2218-1989