Isolierung, enzymatische und immunologische charakterisierung einer 
plasmamembranfraktion vom proximalen tubulus der menschliche niere / Isolation 
and characterization of a plasma membrane fraction from the proximal tubules of 
human kidney

Mondorf, Albert Werner; Kinne, Rolf; Scherberich, Juergen E.; Falkenberg, Frank W.

doi:10.1016/0009-8981(72)90411-1

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Isolierung, enzymatische und immunologische charakterisierung einer plasmamembranfraktion vom proximalen tubulus der menschliche niere / Isolation and characterization of a plasma membrane fraction from the proximal tubules of human kidney

Mondorf, A. W., Kinne, R., Scherberich, J. E., & Falkenberg, F. W. (1972). Isolierung, enzymatische und immunologische charakterisierung einer plasmamembranfraktion vom proximalen tubulus der menschliche niere / Isolation and characterization of a plasma membrane fraction from the proximal tubules of human kidney. Clinica Chimica Acta (Amsterdam), 37, 25-32. doi:10.1016/0009-8981(72)90411-1.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-C244-0 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-C245-F

Genre: Journal Article

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Creators:
Mondorf, Albert Werner¹, Author
Kinne, Rolf², Author
Scherberich, Juergen E.^{1, 2}, Author
Falkenberg, Frank W.^{2, 3}, Author

Affiliations:
1Abteilung für Nephrologie, Zentrum der Inneren Medizin, Goethe-Universität Frankfurt, Frankfurt am Main, Germany, ou_persistent22
2Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297
3Abteilung für Chemie, Lehrstuhl für Biochemie, Ruhr-Universität Bochum, Germany, ou_persistent22

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Abstract: A plasma membrane fraction (PM) from proximal tubules of human kidney cortex was separated by fractionational centrifugation and sucrose density-gradient centrifugation. Marker enzymes, especially from the brush-border were determined. Alkaline phosphatase and an aminopeptidase with alanine specificity were both localized on the membranes. Human anti-PM sera from rabbits revealed—after absorption with human serum proteins—a specific reaction with structures of brush-border. This could be demonstrated by indirect immunofluorescence technique.

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Language(s): deu - German

Dates: Submitted: 1971-06-24Published Online: 2003-01-27Date issued: 1972-03-01

Publication Status: Issued

Pages: 8

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Table of Contents: -

Rev. Type: Peer

Identifiers: DOI: 10.1016/0009-8981(72)90411-1

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Title: Clinica Chimica Acta (Amsterdam)

Other : Clin Chim Acta

Source Genre: Journal

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Publ. Info: Amsterdam : Elsevier

Pages: - Volume / Issue: 37 Sequence Number: - Start / End Page: 25 - 32 Identifier: ISSN: 0009-8981
CoNE: https://pure.mpg.de/cone/journals/resource/954925391288