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  Protein Reconstitution Inside Giant Unilamellar Vesicles

Litschel, T., & Schwille, P. (2021). Protein Reconstitution Inside Giant Unilamellar Vesicles. In Annual Review of Biophysics, Vol 50, 2021 (pp. 525-548).

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 Creators:
Litschel, Thomas1, Author              
Schwille, Petra1, Author              
Affiliations:
1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: MONODISPERSE DOUBLE EMULSIONS; SYNTHETIC CELLS; MEMBRANE-PROTEINS; ACTIN-FILAMENTS; LIPID VESICLES; LIPOSOMES; POLYMERIZATION; DIVISION; MICROTUBULES; EXPRESSIONmodel membranes; synthetic biology; bottom-up biology; in vitro reconstitution; liposomes; phospholipids;
 Abstract: Giant unilamellar vesicles (GUVs) have gained great popularity as mimicries for cellular membranes. As their sizes are comfortably above the optical resolution limit, and their lipid composition is easily controlled, they are ideal for quantitative light microscopic investigation of dynamic processes in and on membranes. However, reconstitution of functional proteins into the lumen or the GUV membrane itself has proven technically challenging. In recent years, a selection of techniques has been introduced that tremendously improve GUV-assay development and enable the precise investigation of protein-membrane interactions under well-controlled conditions. Moreover, due to these methodological advances, GUVs are considered important candidates as protocells in bottom-up synthetic biology. In this review, we discuss the state of the art of the most important vesicle production and protein encapsulation methods and highlight some key protein systems whose functional reconstitution has advanced the field.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published in print
 Pages: 24
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Annual Review of Biophysics, Vol 50, 2021
  Alternative Title : Annu. Rev. Biophys.
  Alternative Title : ANNU REV BIOPHYS
Source Genre: Proceedings
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 525 - 548 Identifier: ISSN: 1936-122X
ISBN: 978-0-8243-1850-5

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Title: Annual Review of Biophysics
  Alternative Title : ANNU REV BIOPHYS
  Alternative Title : Annu. Rev. Biophys.
Source Genre: Series
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: 50 Sequence Number: - Start / End Page: - Identifier: ISSN: 1936-122X