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  Electrochemical Investigations Concerning Ca2+ Binding to Spectrin

Brauer, E., Kupka, K., & Rudloff, V. (1976). Electrochemical Investigations Concerning Ca2+ Binding to Spectrin. Journal of the Electrochemical Society, 123(9), 1313. doi:10.1149/1.2133067.

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 Creators:
Brauer, Elfriede1, Author
Kupka, K.‐D.1, Author
Rudloff, Victor2, Author           
Affiliations:
1Institute of Physical Chemistry, Goethe-University Frankfurt, Frankfurt am Main, Federal Republic of Germany, ou_persistent22              
2Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_3264817              

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Free keywords: calcium binding; bioelectrochemistry; Ca2+-sensitive electrodes, spectrin.
 Abstract: Spectrin, a protein located at the inner surface of the red blood cell membrane, was isolated and investigated with respect to the binding of calcium. Aggregation phenomena were found to be due to the age of spectrin. The electrostatic interaction factor using the Linderstro̸m‐Lang method was found to be very weak, W < 0.01. Calcium binding occurred at 780 sites as indicated by Scatchard plots based on titration curves using a calcium‐sensitive electrode. Titration curves showed in addition the occurrence of conformational changes. The presence of calcium caused the formation of filaments seen in the electron micrographs. The results of binding studies of calcium to spectrin may give information about the influence of calcium on the change of shape of red cell membranes in which spectrin is suggested to be involved.

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Language(s): eng - English
 Dates: 1975-06-301976
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1149/1.2133067
 Degree: -

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Title: Journal of the Electrochemical Society
  Abbreviation : J. Electrochem. Soc.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY, USA : Electrochemical Society
Pages: - Volume / Issue: 123 (9) Sequence Number: - Start / End Page: 1313 Identifier: ISSN: 0013-4651
CoNE: https://pure.mpg.de/cone/journals/resource/991042748197686