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  Direct visualization of amlodipine intervention into living cells by means of fluorescence microscopy

Quentin, C., Gerasimaite, R., Freidzon, A., Atabekyan, L. S., Lukinavičius, G., Belov, V. N., et al. (2021). Direct visualization of amlodipine intervention into living cells by means of fluorescence microscopy. Molecules, 26(10): 2997. doi:10.3390/molecules26102997.

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 Creators:
Quentin, C.1, Author              
Gerasimaite, R.2, Author              
Freidzon, A., Author
Atabekyan, L. S., Author
Lukinavičius, G.2, Author              
Belov, V. N.3, Author              
Mitronova, G.3, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              
2Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              
3Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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Free keywords: amlodipine; fluorescence; live cell imaging; microscopy; lysosomes
 Abstract: Amlodipine, a unique long-lasting calcium channel antagonist and antihypertensive drug, has weak fluorescence in aqueous solutions. In the current paper, we show that direct visualization of amlodipine in live cells is possible due to the enhanced emission in cellular environment. We examined the impact of pH, polarity and viscosity of the environment as well as protein binding on the spectral properties of amlodipine in vitro, and used quantum chemical calculations for assessing the mechanism of fluorescence quenching in aqueous solutions. The confocal fluorescence microscopy shows that the drug readily penetrates the plasma membrane and accumulates in the intracellular vesicles. Visible emission and photostability of amlodipine allow confocal time-lapse imaging and the drug uptake monitoring.

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Language(s): eng - English
 Dates: 2021-05-18
 Publication Status: Published online
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3390/molecules26102997
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Title: Molecules
Source Genre: Journal
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Pages: 15 Volume / Issue: 26 (10) Sequence Number: 2997 Start / End Page: - Identifier: -