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  Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics

Yandrapalli, N., Petit, J., Bäumchen, O., & Robinson, T. (2021). Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics. Communications Chemistry, 4: 100. doi:10.1038/s42004-021-00530-1.

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 Creators:
Yandrapalli, Naresh, Author
Petit, Julien1, Author           
Bäumchen, Oliver1, Author           
Robinson, Tom, Author
Affiliations:
1Group Dynamics of fluid and biological interfaces, Department of Dynamics of Complex Fluids, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063300              

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 Abstract: Microfluidic production of giant lipid vesicles presents a paradigm-shift in the development of
artificial cells. While production is high-throughput and the lipid vesicles are mono-disperse
compared to bulk methods, current technologies rely heavily on the addition of additives such
as surfactants, glycerol and even ethanol. Here we present a microfluidic method for producing
biomimetic surfactant-free and additive-free giant unilamellar vesicles. The versatile
design allows for the production of vesicle sizes ranging anywhere from ~10 to 130 μm with
either neutral or charged lipids, and in physiological buffer conditions. Purity, functionality,
and stability of the membranes are validated by lipid diffusion, protein incorporation, and
leakage assays. Usability as artificial cells is demonstrated by increasing their complexity, i.e.,
by encapsulating plasmids, smaller liposomes, mammalian cells, and microspheres. This
robust method capable of creating truly biomimetic artificial cells in high-throughput will
prove valuable for bottom-up synthetic biology and the understanding of membrane function.

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Language(s): eng - English
 Dates: 2021-06-292021
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1038/s42004-021-00530-1
 Degree: -

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Title: Communications Chemistry
Source Genre: Journal
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Pages: 10 Volume / Issue: 4 Sequence Number: 100 Start / End Page: - Identifier: ISSN: 2399-3669