English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics

Yandrapalli, N., Petit, J., Bäumchen, O., & Robinson, T. (2021). Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics. Communications Chemistry, 4: 100. doi:10.1038/s42004-021-00530-1.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
Article.pdf (Publisher version), 5MB
Name:
Article.pdf
Description:
-
OA-Status:
Gold
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Yandrapalli, Naresh1, Author           
Petit, Julien, Author
Bäumchen, Oliver, Author
Robinson, Tom1, Author           
Affiliations:
1Tom Robinson, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2288691              

Content

show
hide
Free keywords: -
 Abstract: Microfluidic production of giant lipid vesicles presents a paradigm-shift in the development of artificial cells. While production is high-throughput and the lipid vesicles are mono-disperse compared to bulk methods, current technologies rely heavily on the addition of additives such as surfactants, glycerol and even ethanol. Here we present a microfluidic method for producing biomimetic surfactant-free and additive-free giant unilamellar vesicles. The versatile design allows for the production of vesicle sizes ranging anywhere from ~10 to 130 µm with either neutral or charged lipids, and in physiological buffer conditions. Purity, functionality, and stability of the membranes are validated by lipid diffusion, protein incorporation, and leakage assays. Usability as artificial cells is demonstrated by increasing their complexity, i.e., by encapsulating plasmids, smaller liposomes, mammalian cells, and microspheres. This robust method capable of creating truly biomimetic artificial cells in high-throughput will prove valuable for bottom-up synthetic biology and the understanding of membrane function.

Details

show
hide
Language(s): eng - English
 Dates: 2021-06-292021
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Communications Chemistry
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Springer Nature
Pages: - Volume / Issue: 4 Sequence Number: 100 Start / End Page: - Identifier: ISSN: 2399-3669

Source 2

show
hide
Title: bioRxiv
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cold Spring Harbor : Cold Spring Harbor Laboratory
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: -