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  Three-dimensional superresolution fluorescence microscopy maps the variable molecular architecture of the nuclear pore complex

Sabinina, V. J., Hossain, M. J., Hériché, J.-K., Hoess, P., Nijmeijer, B., Mosalaganti, S., et al. (2021). Three-dimensional superresolution fluorescence microscopy maps the variable molecular architecture of the nuclear pore complex. Molecular Biology of the Cell, 32(17), 1523-1533. doi:10.1091/mbc.E20-11-0728.

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 Creators:
Sabinina, Vilma Jimenez1, Author
Hossain, M. Julius1, Author
Hériché, Jean-Karim1, Author
Hoess, Philipp1, 2, Author
Nijmeijer, Bianca1, Author
Mosalaganti, Shyamal1, Author
Kueblbeck, Moritz1, Author
Callegari, Andrea1, Author
Szymborska, Anna3, Author
Beck, Martin1, 4, Author              
Ries, Jonas1, Author
Ellenberg, Jan1, Author
Affiliations:
1Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany, ou_persistent22              
2Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, ou_persistent22              
3Max Delbrück Center for Molecular Medicine, Berlin, Germany, ou_persistent22              
4Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society, ou_3040395              

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 Abstract: Nuclear pore complexes (NPCs) are large macromolecular machines that mediate the traffic between the nucleus and the cytoplasm. In vertebrates, each NPC consists of ∼1000 proteins, termed nucleoporins, and has a mass of over 100 MDa. While a pseudo-atomic static model of the central scaffold of the NPC has recently been assembled by integrating data from isolated proteins and complexes, many structural components still remain elusive due to the enormous size and flexibility of the NPC. Here, we explored the power of 3D super-resolution microscopy combined with computational classification and averaging to explore the 3D structure of the NPC in single human cells. We show that this approach can build the first integrated 3D structural map containing both central as well as peripheral NPC subunits with molecular specificity and nanoscale resolution. Our unbiased classification of over ten thousand individual NPCs indicates that the nuclear ring and the nuclear basket can adopt different conformations. Our approach opens up the exciting possibility to relate different structural states of the NPC to function in situ.

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Language(s): eng - English
 Dates: 2021-04-162020-12-042021-06-212021-06-302021-08-15
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1091/mbc.E20-11-0728
BibTex Citekey: sabinina_3d_2021
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Title: Molecular Biology of the Cell
  Other : Mol. Biol. Cell
Source Genre: Journal
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Publ. Info: American Society for Cell Biology
Pages: - Volume / Issue: 32 (17) Sequence Number: - Start / End Page: 1523 - 1533 Identifier: ISSN: 1059-1524
CoNE: https://pure.mpg.de/cone/journals/resource/954927716372_1