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  An ultracentrifuge study on the self-association of glucose dehydrogenase from Bacillus megaterium

Schubert, D., Maurer, E., Boss, K., & Pfleiderer, G. (1984). An ultracentrifuge study on the self-association of glucose dehydrogenase from Bacillus megaterium. Hoppe-Seyler's Zeitschrift für physiologische Chemie, 365(12), 1445-1449. doi:10.1515/bchm2.1984.365.2.1445.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-CD53-4 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-CD54-3
Genre: Journal Article

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 Creators:
Schubert, Dieter1, Author           
Maurer, Eberhard2, Author
Boss, Karin2, Author
Pfleiderer, Gerhard2, Author
Affiliations:
1Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_3264817              
2Institut für Organische Chemie, Biochemie und Isotopenforschung der Universität Stuttgart, Germany, ou_persistent22              

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Free keywords: Glucose dehydrogenase; monomer/dimer/tetramer association equilibrium; analytical ultracentrifugation; Bacillus mega
 Abstract: The self-association of glucose dehydrogenase (beta-D-glucose:NAD(P) 1-oxidoreductase, EC 1.1.1.47) from Bacillus megaterium was studied by analytical ultracentrifugation. The pH and composition of the buffer used were such that, owing to a reversible partial dissociation of the tetrameric enzyme, enzyme activity was reduced. It was found that under these conditions the protein exists in a monomer/dimer/tetramer association equilibrium.

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Language(s): eng - English
 Dates: 1984-08-011984-12-01
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1515/bchm2.1984.365.2.1445
PMID: 6441770
 Degree: -

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Title: Hoppe-Seyler's Zeitschrift für physiologische Chemie
  Other : Biol. Chem. Hoppe-Seyler
Source Genre: Journal
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Publ. Info: Berlin : W. de Gruyter
Pages: - Volume / Issue: 365 (12) Sequence Number: - Start / End Page: 1445 - 1449 Identifier: ISSN: 0177-3593
CoNE: https://pure.mpg.de/cone/journals/resource/954925623257