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  Structure-based insights into evolution of rhodopsins

Zabelskii, D., Dmitrieva, N., Volkov, O., Shevchenko, V., Kovalev, K., Balandin, T., et al. (2021). Structure-based insights into evolution of rhodopsins. Communications Biology, 4(1): 821. doi:10.1038/s42003-021-02326-4.

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Zabelskii, Dmitrii1, Author
Dmitrieva, Natalia1, Author
Volkov, Oleksandr1, Author
Shevchenko, Vitaly1, Author
Kovalev, Kirill1, Author
Balandin, Taras1, Author
Soloviov, Dmytro1, Author
Astashkin, Roman1, Author
Zinovev, Egor1, Author
Alekseev, Alexey1, Author
Round, Ekaterina1, Author
Polovinkin, Vitaly1, Author
Chizhov, Igor1, Author
Rogachev, Andrey1, Author
Okhrimenko, Ivan1, Author
Borshchevskiy, Valentin1, Author
Chupin, Vladimir1, Author
Büldt, Georg1, Author
Yutin, Natalia1, Author
Bamberg, Ernst2, Author              
Koonin, Eugene1, AuthorGordeliy, Valentin1, Author more..
Affiliations:
1External Organizations, ou_persistent22              
2Emeritusgroup Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2253652              

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 Abstract: Rhodopsins, most of which are proton pumps generating transmembrane electrochemical proton gradients, span all three domains of life, are abundant in the biosphere, and could play a crucial role in the early evolution of life on earth. Whereas archaeal and bacterial proton pumps are among the best structurally characterized proteins, rhodopsins from unicellular eukaryotes have not been well characterized. To fill this gap in the current understanding of the proton pumps and to gain insight into the evolution of rhodopsins using a structure-based approach, we performed a structural and functional analysis of the light-driven proton pump LR (Mac) from the pathogenic fungus Leptosphaeria maculans. The first high-resolution structure of fungi rhodopsin and its functional properties reveal the striking similarity of its membrane part to archaeal but not to bacterial rhodopsins. We show that an unusually long N-terminal region stabilizes the protein through direct interaction with its extracellular loop (ECL2). We compare to our knowledge all available structures and sequences of outward light-driven proton pumps and show that eukaryotic and archaeal proton pumps, most likely, share a common ancestor.

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Language(s): eng - English
 Dates: 2020-10-072021-06-072021-06-30
 Publication Status: Published online
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s42003-021-02326-4
BibTex Citekey: zabelskii_structure-based_2021
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Title: Communications Biology
Source Genre: Journal
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Publ. Info: London : Springer Nature
Pages: - Volume / Issue: 4 (1) Sequence Number: 821 Start / End Page: - Identifier: ISSN: 2399-3642
CoNE: https://pure.mpg.de/cone/journals/resource/2399-3642