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キーワード:
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要旨:
Conversion of biological feedstocks into value-added chemicals is mostly
performed via microbial fermentation. An emerging alternative approach
is the use of cell-free systems, consisting of purified enzymes and
cofactors. Unfortunately, the in vivo and in vitro research communities
rarely interact, which leads to oversimplifications and exaggerations
that do not permit fair comparison of the two strategies and impede
synergistic interactions. Here, we provide a comprehensive account for
the advantages and drawbacks associated with each strategy, and further
discuss recent research efforts that aim to breach the limits of
cellular and cell-free production. We also explore emerging hybrid
solutions that integrate the benefits of both worlds and could expand
the boundaries of biosynthesis.
