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  3D particle averaging and detection of macromolecular symmetry in localization microscopy

Heydarian, H., Joosten, M., Przybylski, A., Schueder, F., Jungmann, R., van Werkhoven, B., et al. (2021). 3D particle averaging and detection of macromolecular symmetry in localization microscopy. Nature Communications, 12: 2847. doi:10.1038/s41467-021-22006-5.

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 Creators:
Heydarian, Hamidreza1, Author
Joosten, Maarten1, Author
Przybylski, Adrian1, Author
Schueder, Florian2, Author           
Jungmann, Ralf2, Author           
van Werkhoven, Ben1, Author
Keller-Findeisen, Jan1, Author
Ries, Jonas1, Author
Stallinga, Sjoerd1, Author
Bates, Mark1, Author
Rieger, Bernd1, Author
Affiliations:
1external, ou_persistent22              
2Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              

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Free keywords: SINGLE-MOLECULE LOCALIZATION; SUPERRESOLUTION MICROSCOPY; RESOLUTION; RESOLVESScience & Technology - Other Topics;
 Abstract: Single molecule localization microscopy offers in principle resolution down to the molecular level, but in practice this is limited primarily by incomplete fluorescent labeling of the structure. This missing information can be completed by merging information from many structurally identical particles. In this work, we present an approach for 3D single particle analysis in localization microscopy which hugely increases signal-to-noise ratio and resolution and enables determining the symmetry groups of macromolecular complexes. Our method does not require a structural template, and handles anisotropic localization uncertainties. We demonstrate 3D reconstructions of DNA-origami tetrahedrons, Nup96 and Nup107 subcomplexes of the nuclear pore complex acquired using multiple single molecule localization microscopy techniques, with their structural symmetry deducted from the data. Adaptation of current algorithms to 3D SMLM data is currently problematic. Here the authors report a method that increases the signal-to-noise ratio and resolution of 3D single particle analysis in localization microscopy and enables determination of the symmetry groups of macromolecular complexes.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 12 Sequence Number: 2847 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723