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  Deep muscle-proteomic analysis of freeze-dried human muscle biopsies reveals fiber type-specific adaptations to exercise training

Deshmukh, A. S., Steenberg, D. E., Hostrup, M., Birk, J. B., Larsen, J. K., Santos, A., et al. (2021). Deep muscle-proteomic analysis of freeze-dried human muscle biopsies reveals fiber type-specific adaptations to exercise training. Nature Communications, 12(1): 304. doi:10.1038/s41467-020-20556-8.

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 Creators:
Deshmukh, A. S.1, Author
Steenberg, D. E.1, Author
Hostrup, M.1, Author
Birk, J. B.1, Author
Larsen, J. K.1, Author
Santos, A.1, Author
Kjobsted, R.1, Author
Hingst, J. R.1, Author
Scheele, C. C.1, Author
Murgia, M.2, Author              
Kiens, B.1, Author
Richter, E. A.1, Author
Mann, M.2, Author              
Wojtaszewski, J. F. P.1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: HUMAN SKELETAL-MUSCLE; AMPK; GLUCOSE-6-PHOSPHATE-DEHYDROGENASE; COMPLEMENT; METABOLISM; EXTRACTION; GLYCOGENIN; CYTOSCAPE; PROTEINS; ISOFORMSScience & Technology - Other Topics;
 Abstract: Skeletal muscle conveys several of the health-promoting effects of exercise; yet the underlying mechanisms are not fully elucidated. Studying skeletal muscle is challenging due to its different fiber types and the presence of non-muscle cells. This can be circumvented by isolation of single muscle fibers. Here, we develop a workflow enabling proteomics analysis of pools of isolated muscle fibers from freeze-dried human muscle biopsies. We identify more than 4000 proteins in slow- and fast-twitch muscle fibers. Exercise training alters expression of 237 and 172 proteins in slow- and fast-twitch muscle fibers, respectively. Interestingly, expression levels of secreted proteins and proteins involved in transcription, mitochondrial metabolism, Ca2+ signaling, and fat and glucose metabolism adapts to training in a fiber type-specific manner. Our data provide a resource to elucidate molecular mechanisms underlying muscle function and health, and our workflow allows fiber type-specific proteomic analyses of snap-frozen non-embedded human muscle biopsies.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 12 (1) Sequence Number: 304 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723