Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

 
 
DownloadE-Mail
  ESCRT-III induces phase separation in model membranes prior to budding and causes invagination of the liquid-ordered phase

Avalos Padilla, Y., Georgiev, V., & Dimova, R. (2021). ESCRT-III induces phase separation in model membranes prior to budding and causes invagination of the liquid-ordered phase. Biochimica et Biophysica Acta: BBA, 1863(10): 183689. doi:10.1016/j.bbamem.2021.183689.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
Publisher PDF.pdf (Verlagsversion), 2MB
 
Datei-Permalink:
-
Name:
Publisher PDF.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
Movie S3.avi (Ergänzendes Material), 393KB
 
Datei-Permalink:
-
Name:
Movie S3.avi
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Typ / Prüfsumme:
video/x-msvideo
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
Movie S1.mp4 (Ergänzendes Material), 793KB
 
Datei-Permalink:
-
Name:
Movie S1.mp4
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Typ / Prüfsumme:
video/mp4
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
Movie S2.avi (Ergänzendes Material), 607KB
 
Datei-Permalink:
-
Name:
Movie S2.avi
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Typ / Prüfsumme:
video/x-msvideo
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
Author Manuscript.pdf (beliebiger Volltext), 3MB
Name:
Author Manuscript.pdf
Beschreibung:
-
OA-Status:
Keine Angabe
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Avalos Padilla, Yunuen1, Autor           
Georgiev, Vasil1, Autor           
Dimova, Rumiana1, Autor           
Affiliations:
1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              

Inhalt

einblenden:
ausblenden:
Schlagwörter: ESCRT-III Phase separation Lipid domains GUVs Ternary mixtures Membrane fission Microcompartments
 Zusammenfassung: Membrane fission triggered by the endosomal sorting complex required for transport (ESCRT) is an important process observed in several pathogenic and non-pathogenic cellular events. From a synthetic-biology viewpoint, ESCRT proteins represent an interesting machinery for the construction of cell mimetic sub-compartments produced by fission. Since their discovery, the studies on ESCRT-III-mediated action, have mainly focused on protein dynamics, ignoring the role of lipid organization and membrane phase state. Recently, it has been suggested that membrane buds formed by the action of ESCRT-III are generated from transient microdomains in endosomal membranes. However, the interplay between membrane domain formation and ESCRT remodeling pathways has not been investigated. Here, giant unilamellar vesicles made of ternary lipid mixtures, either homogeneous in phase or exhibiting liquid-ordered/liquid-disordered phase coexistence, were employed as a model membrane system. These vesicles were incubated with purified recombinant ESCRT-III proteins from the parasite Entamoeba histolytica. In homogeneous membranes, we observe that EhVps32 can trigger domain formation while EhVps20 preferentially co-localizes in the liquid disordered phase. The addition of EhVps24 appears to induce the formation of intraluminal vesicles produced from the liquid-ordered phase. In phase separated membranes, the intraluminal vesicles are also generated from the liquid-ordered phase and presumably emerge from the phase boundary region. Our findings reinforce the hypothesis that ESCRT-mediated remodeling depends on the membrane phase state. Furthermore, the obtained results point to a potential synthetic biology approach for establishing eukaryotic mimics of artificial cells with microcompartments of specific membrane composition, which can also differ from that of the mother vesicle.

Details

einblenden:
ausblenden:
Sprache(n):
 Datum: 2021-07-022021
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: DOI: 10.1016/j.bbamem.2021.183689
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Biochimica et Biophysica Acta : BBA
  Andere : Biochimica et Biophysica Acta (BBA) - Biomembranes
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Amsterdam : Elsevier
Seiten: - Band / Heft: 1863 (10) Artikelnummer: 183689 Start- / Endseite: - Identifikator: ISSN: 1879-2642