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  Pressure effects on the interactions of the sarcoplasmic reticulum calcium transport enzyme with calcium and para-nitrophenyl phosphate

Hasselbach, W., & Stephan, L. (1987). Pressure effects on the interactions of the sarcoplasmic reticulum calcium transport enzyme with calcium and para-nitrophenyl phosphate. Zeitschrift für Naturforschung, C: Journal of Biosciences, 42(5), 641-652.

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 Creators:
Hasselbach, Wilhelm1, Author           
Stephan, Lore1, Author           
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1Department of Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_3321127              

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 Abstract: The effect of hydrostatic pressure on calcium dependent p-nitrophenyl phosphate hydrolysis of the sarcoplasmic reticulum calcium transport enzyme has been investigated at different degree of enzyme saturation by calcium and Mg-p-nitrophenyl phosphate to distinguish between activation and binding volumes. The enzyme saturated by both ligands displays a significant dependence of the activation volume on pressure, rising from 20 ml/mol at atmospheric pressure (0.1 MPa) to 80 ml/mol at 100 MPa. At subsaturating concentration of Mg-p-nitrophenyl phosphate an activation volume of 35 ml/mol prevails between 0.1 and 40 MPa. At subsaturating concentration of calcium the activation volume approximates 80 ml/mol in the same pressure range. The binding volume for both substrates is likewise pressure dependent falling from 20 ml/mol to 0 ml/mol for Mg-p-nitrophenyl phosphate and rising from 67 ml/mol to 155 ml/mol for calcium. The pressure dependence of activation and binding volumes is analysed on account of a simplified reaction scheme yielding activation volumes and rate constants for individual reaction steps.

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Language(s): eng - English
 Dates: 1987-05-01
 Publication Status: Issued
 Pages: 12
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: PMID: 2955599
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Title: Zeitschrift für Naturforschung, C: Journal of Biosciences
  Abbreviation : Z. Naturforsch., C: J. Biosci.
Source Genre: Journal
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Publ. Info: Berlin : Walter de Gruyter GmbH
Pages: - Volume / Issue: 42 (5) Sequence Number: - Start / End Page: 641 - 652 Identifier: ISSN: 1865-7125
CoNE: https://pure.mpg.de/cone/journals/resource/954927655916_1