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  Light-triggered cargo loading and division of DNA-containing giant unilamellar lipid vesicles

Dreher, Y., Jahnke, K., Schröter, M., & Göpfrich, K. (2021). Light-triggered cargo loading and division of DNA-containing giant unilamellar lipid vesicles. Nano Letters, 21(14), 5952-5957. doi:10.1021/acs.nanolett.1c00822.

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 Creators:
Dreher, Yannik1, Author              
Jahnke, Kevin1, Author              
Schröter, Martin1, Author              
Göpfrich, Kerstin1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: Giant unilamellar lipid vesicle (GUV); synthetic cell division; lipid peroxidation; cargo loading; Chlorin e6 (Ce6)
 Abstract: A minimal synthetic cell should contain a substrate for information storage and have the capability to divide. Notable efforts were made to assemble functional synthetic cells from the bottom up, however often lacking the capability to reproduce. Here, we develop a mechanism to fully control reversible cargo loading and division of DNA-containing giant unilamellar vesicles (GUVs) with light. We make use of the photosensitizer Chlorin e6 (Ce6) which self-assembles into lipid bilayers and leads to local lipid peroxidation upon illumination. On the time scale of minutes, illumination induces the formation of transient pores, which we exploit for cargo encapsulation or controlled release. In combination with osmosis, complete division of two daughter GUVs can be triggered within seconds of illumination due to a spontaneous curvature increase. We ultimately demonstrate the division of a selected DNA-containing GUV with full spatiotemporal control—proving the relevance of the division mechanism for bottom-up synthetic biology.

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Language(s): eng - English
 Dates: 2021-07-022021-02-262021-07-122021-07-122021-07-28
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Nano Letters
  Abbreviation : Nano Lett.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 21 (14) Sequence Number: - Start / End Page: 5952 - 5957 Identifier: ISSN: 1530-6984
CoNE: https://pure.mpg.de/cone/journals/resource/110978984570403