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  A method for the temperature-controlled extraction of DNA from ancient bones

Essel, E., Korlević, P., & Meyer, M. (2021). A method for the temperature-controlled extraction of DNA from ancient bones. Biotechniques, 71(1), 383-387. doi:10.2144/btn-2021-0025.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-E0F5-6 Version Permalink: https://hdl.handle.net/21.11116/0000-000B-F9A5-2
Genre: Journal Article

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Essel_Method_BioTechniques_2021.pdf (Publisher version), 298KB
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https://hdl.handle.net/21.11116/0000-0008-E0F7-4
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Essel_Method_BioTechniques_2021.pdf
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2021
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This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
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http://creativecommons.org/licenses/by-nc-nd/4.0/

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https://www.protocols.io/view/a-method-for-the-temperature-controlled-extraction-bje5kjg6 (Supplementary material)
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 Creators:
Essel, Elena1, Author                 
Korlević, Petra1, Author           
Meyer, Matthias1, Author           
Affiliations:
1Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society, Deutscher Platz 6, 04103 Leipzig, DE, ou_2074332              

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Free keywords: Ancient DNA, sequential DNA extraction, contamination removal, endogenous DNA, archaeological material
 Abstract: We here provide a protocol for the decontamination of ancient bones and teeth that is based on a temperature-controlled, sequential release of DNA. DNA can be extracted from all fractions generated with this method and the fraction with the highest proportion of endogenous DNA identified for further analysis. The protocol proceeds through repeated incubation of the sample powder in phosphate buffer at 37, 60 and 90 °C, followed by the complete lysis of the residual sample powder. As DNA is denatured at high temperature, subsequent DNA extraction and library preparation has to be performed using methods optimized for single-stranded DNA.

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Language(s): eng - English
 Dates: 2021-06-07
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.2144/btn-2021-0025
 Degree: -

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Title: Biotechniques
Source Genre: Journal
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Pages: - Volume / Issue: 71 (1) Sequence Number: - Start / End Page: 383 - 387 Identifier: -