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  Laser Scanning Microscopy To Study Molecular Transport In Single Cells

Scholz, M., Sauer, H., Rihs, H.-P., & Peters, R. (1989). Laser Scanning Microscopy To Study Molecular Transport In Single Cells. Proceedings of SPIE, 1028, 160-166.

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Genre: Conference Paper

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 Creators:
Scholz, Manfred1, Author              
Sauer, Heinrich1, Author              
Rihs, Hans-Peter1, Author              
Peters, Reiner1, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_3264817              

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Free keywords: Luminescence; Proteins; Laser scanners; Microscopy; Microscopes; Diffusion
 Abstract: Laser scanning microscopy was used to study dynamic processes in single cells. The laser scanning microscope of Heidelberg Instruments was complemented with a 1W krypton laser and a microinjection set-up. Simple algorithms were worked out which permit determination of local and integrated intensities in fluorescence scans. In one application the lateral diffusion of macromolecules was studied. The krypton laser was used to irreversibly photolyse fluorescently labeled dextrans in small volumes of a thin fluid layer; equilibration of the local fluorescence inhomogeneity by lateral diffusion was followed by repetitive scanning. In a second application the permeability of single red blood cell membranes which had been exposed to the complement cascade was studied. In a further application artificial nuclear proteins, constructed by molecular genetic methods, were injected into the cytoplasm of hepatoma cells. The kinetics of protein transport from cytoplasm to nucleus were derived from fluorescence scans. In all applications a good agreement between results obtained by laser scanning microscopy and those obtained independently by other methods and instruments was observed.

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Language(s): eng - English
 Dates: 1989-02-09
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1117/12.950335
 Degree: -

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Title: International Congress on Optical Science and Engineering
Place of Event: Hamburg, Germany
Start-/End Date: 1988

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Title: Proceedings of SPIE
  Other : Society of Photo-Optical Instrumentation Engineers (SPIE)
Source Genre: Journal
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Publ. Info: Bellingham, Washington : SPIE
Pages: - Volume / Issue: 1028 Sequence Number: - Start / End Page: 160 - 166 Identifier: ISSN: 0277-786X
CoNE: https://pure.mpg.de/cone/journals/resource/0277-786X