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  Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome

Runge, A. K. W., Hendy, J., Richter, K. K., Masson-MacLean, E., Britton, K., Mackie, M., et al. (2021). Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome. Proceedings of the Royal Society B: Biological Sciences, 288(1954): 20210020, pp. 1-10. doi:10.1098/rspb.2021.0020.

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 Creators:
Runge, Anne Kathrine W., Author
Hendy, Jessica1, Author              
Richter, Kristine Korzow2, Author              
Masson-MacLean, Edouard, Author
Britton, Kate, Author              
Mackie, Meaghan, Author
McGrath, Krista, Author
Collins, Matthew, Author
Cappellini, Enrico, Author
Speller, Camilla, Author
Affiliations:
1Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074310              
2Archaeology, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074312              

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Free keywords: Nunalleq Alaska, zooms, palaeoproteomics, palaeofaeces, archaeology, dogs
 Abstract: The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply zooarchaeology by mass spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes.

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Language(s): eng - English
 Dates: 2021-07-072021-07-14
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: 1. Introduction
2. Material and methods
(a) Samples
(b) Palaeoproteomics
(c) Zooarchaeology by mass spectrometry
3. Results and discussion
(a) Metaproteomics
(b) Host proteins
(c) Dietary proteins
(d) Challenges and future directions
4. Conclusion
 Rev. Type: Peer
 Identifiers: DOI: 10.1098/rspb.2021.0020
Other: shh2991
 Degree: -

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Title: Proceedings of the Royal Society B: Biological Sciences
  Abbreviation : Proc. R. Soc. B
Source Genre: Journal
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Publ. Info: London : Royal Society
Pages: - Volume / Issue: 288 (1954) Sequence Number: 20210020 Start / End Page: 1 - 10 Identifier: ISSN: 0962-8452
CoNE: https://pure.mpg.de/cone/journals/resource/110975500577295_2